Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells.

Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Research Abstract Details 

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Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Abstract Text:

M A Axelsson,E M Hansson,R Sikut,G C Hansson,

Strongly reacting antibodies specific for defined mucin gene products are often directed against the mucin protein backbone of the heavily glycosylated serine/threonine rich regions. A prerequisite for the use of such antibodies is often the complete removal of the oligosaccharides from the protein. This paper describes an efficient one-step deglycosylation method using gaseous hydrogen fluoride on nylon blotting membranes and microtiter wells.

Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Publishing Authors By Initials

MA Axelsson,EM Hansson,R Sikut,GC Hansson,

For similar carbohydrates: polysaccharides: oligosaccharides research abstracts see: carbohydrates: polysaccharides: oligosaccharides research

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Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Glycoconjugate journal

VOLUME: 15

Page Numbers: 749-55

Journal Abbreviation: Glycoconj. J.

ISSN: 0282-0080

DAY: 1

MONTH: Aug

YEAR: 1998

Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Information

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LANGUAGE: eng

NlmUniqueID: 8603310

Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Keywords Mesh Terms:

KEYWORDS: Oligosaccharides

MESH TERMS: immunology

Chemical & Substance for Abstract: Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells. Information

Substance Name: Hydrofluoric Acid

Registry Number: 7664-39-3

Grant and Affiliation Information for Deglycosylation by gaseous hydrogen fluoride of mucus glycoproteins immobilized on nylon membranes and in microtiter wells.

AFFILIATION: Department of Medical Biochemistry, University of Göteborg, Gothenburg, Sweden.

Country: ENGLAND

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MEDLINETA: Glycoconj J

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