Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1.

Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1. Abstract Text:

G A Pietersz,W Li,C Osinski,V Apostolopoulos,I F McKenzie,

Mucin1 (MUC1) is expressed ubiquitously on breast cancer cells and is a potential target for the generation of cytotoxic T cells for vaccination against breast cancer. Thus far studies of the immunogenicity of MUC1 have used peptides from the variable number of tandem repeat (VNTR); mice so immunised can generate strong cellular and antibody responses to the VNTR of human MUC1. We now demonstrate that significant CTL and CTLp can be induced to other regions of MUC1. Using the whole native MUC1 molecule, the human milk fat globule membrane antigen (HMFG) linked to mannan, cytotoxic T cell precursors (CTLp) can be generated in BALB/c, C57BL/6, transgenic HLA-A*0201/K(b) and double transgenic HLA-A*0201/K(b)xhuman MUC1 (A2 K(b)MUC1) mice. By immunising with HMFG and testing selectively on (a) extracellular (non-VNTR); (b) VNTR and (c) intracellular peptides, it was shown that all three regions generated effective CTL. Further, the CTL responses to non-VNTR peptides were as strong as those generated to the VNTR. Epitope prediction algorithms were not particularly helpful to describe CTL epitopes: overlapping peptides had to be synthesised and tested to find the epitopes. Thus, for CTL generation, the whole HMFG molecule is a powerful immunogen when linked to mannan, especially as multiple peptide epitopes for presentation by many Class I molecules are contained within the one molecule. Furthermore, Class I restricted MUC1 CTL were generated in double transgenic A2 K(b)MUC1 mice by immunising with mannan-native mucin (HMFG), suggesting that tolerance to MUC1 can be overcome with mannan-HMFG.

Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1. Publishing Authors By Initials

GA Pietersz,W Li,C Osinski,V Apostolopoulos,IF McKenzie,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: repetitive sequences, nucleic acid: tandem repeat sequences research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: molecular structure: base sequence: repetitive sequences, nucleic acid: tandem repeat sequences research

PUBMED ID PMID:

MEDLINE DATE:

Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Vaccine

VOLUME: 18

Page Numbers: 2059-71

Journal Abbreviation: Vaccine

ISSN: 0264-410X

DAY: 3

MONTH: Apr

YEAR: 2000

Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8406899

Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1. Keywords Mesh Terms:

KEYWORDS: Tandem Repeat Sequences

MESH TERMS: immunology

Chemical & Substance for Abstract: Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1. Information

Substance Name: milk fat globule

Registry Number: 0

Grant and Affiliation Information for Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1.

AFFILIATION: The Austin Research Institute, Studley Rd, Heidelberg, Australia. g.pietersz@ari.unimelb.edu.au

Country: ENGLAND

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Vaccine

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1 Related Publications

• A carbohydrate/protein energy gel improves swimming performance in collegiate swimmers: 2061: board #131 may 31 3:30 PM - 5:00 PM.
• A stochastic model of immune-modulated malaria infection and disease in children.
• Affordable care today, not a world away.
• Aldehyde-mannan antigen complexes target the MHC class I antigen-presentation pathway.
• Balancing costs and benefits of checking in radiotherapy.
• Breast cancer in mice: effect of murine MUC-1 immunization on tumor incidence in C3H/HeOuj mice.
• Comparison of Gastrocnemius and Soleus Muscle Gene Responses to an Acute Aerobic Run: 2499: Board #46 June 2 9:30 AM - 11:00 AM.
• Definition of MHC-restricted CTL epitopes from non-variable number of tandem repeat sequence of MUC1.
• ELDONET-A Decade of Monitoring Solar Radiation on Five Continents.
• Environmental constraints upon locomotion and predator-prey interactions in aquatic organisms: an introduction.
• Expression of MUC1 and MUC2 mucins by human tumor cell lines.
• How to use 'Total-QALYs' to Make the Treatment Justifications Required by IRMER.
• Influx of neutrophils and persistence of cytokine expression in airways of horses after performing exercise while breathing cold air.
• Lung density is not altered following intense normobaric hypoxic interval training in competitive female cyclists.
• MUC1 and breast cancer.
• Monoclonal antibodies to mucin VNTR peptides.
• Natural human anti-Gal alpha(1,3)Gal antibodies react with human mucin peptides.
• Notes on the Dissection of Two Club Feet.
• Peptide binding sites recognized by anti-mucin (MUC2) monoclonal antibodies.
• Peptide epitopes in breast cancer mucins.
• Physical Activity and Academic Achievement: 119: 3:25 PM-3:55 PM.
• Poisoning of companion animals by garden and house plants in Queensland: a veterinary practice survey.
• Polyuria and polydipsia in horses.
• Prothrombotic factors enhance heparin-induced thrombocytopenia and thrombosis in vivo in a mouse model.
• Reader technique as a source of variability in determining malaria parasite density by microscopy.
• Reproducibility of a Laboratory Based 20-km Time Trial Evaluation in Competitive Cyclists Using the Velotron Pro Ergometer.
• The Effect of Arm Cranking Exercise on Lymphatic Function in Breast Cancer Survivors with and without Lymphedema: 791: June 1 9:15 AM - 9:30 AM.
• Tracing increasing tropical Andean glacier melt with stable isotopes in water.
• Upper extremity lymphatic function at rest and during exercise in breast cancer survivors with and without lymphedema compared with healthy controls.
• Using agar plates to teach the importance of hand hygiene.