Definition and spatial annotation of the dynamic secretome during early kidney development.

Definition and spatial annotation of the dynamic secretome during early kidney development. Research Abstract Details 

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Definition and spatial annotation of the dynamic secretome during early kidney development. Abstract Text:

Gemma Martinez,Kylie Georgas,Grant A Challen,Bree Rumballe,Melissa J Davis,Darrin Taylor,Rohan D Teasdale,Sean M Grimmond,Melissa H Little,

The term "secretome" has been defined as a set of secreted proteins (Grimmond et al. [2003] Genome Res 13:1350-1359). The term "secreted protein" encompasses all proteins exported from the cell including growth factors, extracellular proteinases, morphogens, and extracellular matrix molecules. Defining the genes encoding secreted proteins that change in expression during organogenesis, the dynamic secretome, is likely to point to key drivers of morphogenesis. Such secreted proteins are involved in the reciprocal interactions between the ureteric bud (UB) and the metanephric mesenchyme (MM) that occur during organogenesis of the metanephros. Some key metanephric secreted proteins have been identified, but many remain to be determined. In this study, microarray expression profiling of E10.5, E11.5, and E13.5 kidney and consensus bioinformatic analysis were used to define a dynamic secretome of early metanephric development. In situ hybridisation was used to confirm microarray results and clarify spatial expression patterns for these genes. Forty-one secreted factors were dynamically expressed between the E10.5 and E13.5 timeframe profiled, and 25 of these factors had not previously been implicated in kidney development. A text-based anatomical ontology was used to spatially annotate the expression pattern of these genes in cultured metanephric explants.

Definition and spatial annotation of the dynamic secretome during early kidney development. Publishing Authors By Initials

G Martinez,K Georgas,GA Challen,B Rumballe,MJ Davis,D Taylor,RD Teasdale,SM Grimmond,MH Little,

For similar investigative techniques: clinical laboratory techniques: culture techniques: tissue culture techniques research abstracts see: investigative techniques: clinical laboratory techniques: culture techniques: tissue culture techniques research

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Definition and spatial annotation of the dynamic secretome during early kidney development. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Developmental dynamics : an official publication o

VOLUME: 235

Page Numbers: 1709-19

Journal Abbreviation: Dev. Dyn.

ISSN: 1058-8388

DAY: 3

MONTH: Jun

YEAR: 2006

Definition and spatial annotation of the dynamic secretome during early kidney development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 9201927

Definition and spatial annotation of the dynamic secretome during early kidney development. Keywords Mesh Terms:

KEYWORDS: Tissue Culture Techniques

MESH TERMS: secretion

Chemical & Substance for Abstract: Definition and spatial annotation of the dynamic secretome during early kidney development. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for Definition and spatial annotation of the dynamic secretome during early kidney development.

AFFILIATION: Institute for Molecular Bioscience, The University of Queensland, Queensland, Australia.

Country: United States

AGENCY: United States NIDDK

GRANT: DK63400

ACRONYM: DK

MEDLINETA: Dev Dyn

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