Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli.

Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Research Abstract Details 

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Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Abstract Text:

Joseph G Sklar,Tao Wu,Daniel Kahne,Thomas J Silhavy,

Integral beta-barrel proteins (OMPs) are a major class of outer membrane proteins in Gram-negative bacteria. In Escherichia coli, these proteins are synthesized in the cytoplasm, translocated across the inner membrane via the Sec machinery, and assembled in the outer membrane through an unknown mechanism that requires the outer membrane YaeT complex and the periplasmic chaperones SurA, DegP, and Skp. Here, we have established the relationship between these three chaperones providing insight into the mechanism of OMP biogenesis using depletion analysis. Depletion of SurA alone results in a marked decrease in outer membrane density, while the loss of DegP and Skp has no effect on outer membrane composition. Furthermore, we demonstrate that SurA and YaeT interact directly in vivo. Based on these results, we suggest that SurA is the primary chaperone responsible for the periplasmic transit of the bulk mass of OMPs to the YaeT complex. The role of Skp and DegP is amplified in the absence of SurA. Evidence presented suggests that DegP/Skp function to rescue OMPs that fall off the SurA pathway. The seemingly redundant periplasmic chaperones do function in parallel, but the relative importance of the primary function of each pathway depends on whether or not cells are under stress.

Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Publishing Authors By Initials

JG Sklar,T Wu,D Kahne,TJ Silhavy,

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Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Genes & development

VOLUME: 21

Page Numbers: 2473-84

Journal Abbreviation: Genes Dev.

ISSN: 0890-9369

DAY: 1

MONTH: Oct

YEAR: 2007

Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli. Information

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LANGUAGE: eng

NlmUniqueID: 8711660

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Grant and Affiliation Information for Defining the roles of the periplasmic chaperones SurA, Skp, and DegP in Escherichia coli.

AFFILIATION: Department of Molecular Biology, Princeton University, Princeton, New Jersey 08544, USA.

Country: United States

AGENCY: United States PHS

GRANT: T32JM07388

ACRONYM: GM

MEDLINETA: Genes Dev

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