Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils.

Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Research Abstract Details 

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Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Abstract Text:

Mai Zhang,Takashi Angata,Jae Youn Cho,Marina Miller,David H Broide,Ajit Varki,

CD33-related Siglecs (CD33rSiglecs) are a family of sialic acid-recognizing lectins on immune cells whose biologic functions are unknown. We studied in vivo functions of Siglec-F, the CD33rSiglec expressed on mouse eosinophils, which are prominent in allergic processes. Induction of allergic lung inflammation in mice caused up-regulation of Siglec-F on blood and bone marrow eosinophils, accompanied by newly induced expression on some CD4(+) cells, as well as quantitative up-regulation of endogenous Siglec-F ligands in the lung tissue and airways. Taken together with the tyrosine-based inhibitory motif in the cytosolic tail of Siglec-F, the data suggested a negative feedback loop, controlling allergic responses of eosinophils and helper T cells, via Siglec-F and Siglec-F ligands. To pursue this hypothesis, we created Siglec-F-null mice. Allergen-challenged null mice showed increased lung eosinophil infiltration, enhanced bone marrow and blood eosinophilia, delayed resolution of lung eosinophilia, and reduced peribronchial-cell apoptosis. Anti-Siglec-F antibody cross-linking also enhanced eosinophil apoptosis in vitro. These data support the proposed negative feedback role for Siglec-F, represent the first in vivo demonstration of biologic functions for any CD33rSiglec, and predict a role for human Siglec-8 (the isofunctional paralog of mouse Siglec-F) in regulating the pathogenesis of human eosinophil-mediated disorders.

Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Publishing Authors By Initials

M Zhang,T Angata,JY Cho,M Miller,DH Broide,A Varki,

For similar cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research abstracts see: cells: blood cells: leukocytes: leukocytes, mononuclear: lymphocytes: t-lymphocytes research

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Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Blood

VOLUME: 109

Page Numbers: 4280-7

Journal Abbreviation:

ISSN: 0006-4971

DAY: 1

MONTH: 02

YEAR: 2007

Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7603509

Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Keywords Mesh Terms:

KEYWORDS: T-Lymphocytes

MESH TERMS: metabolism

Chemical & Substance for Abstract: Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils. Information

Substance Name: N-Acetylneuraminic Acid

Registry Number: 131-48-6

Grant and Affiliation Information for Defining the in vivo function of Siglec-F, a CD33-related Siglec expressed on mouse eosinophils.

AFFILIATION: Glycobiology Research and Training Center, Departments of Medicine and Cellular & Molecular Medicine, and Biomedical Sciences Graduate Program, University of California at San Diego, La Jolla, CA 92093-0687, USA.

Country: United States

AGENCY: United States NHLBI

GRANT: HL57345

ACRONYM: HL

MEDLINETA: Blood

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