Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli.

Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli. Research Abstract Details 

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Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli. Abstract Text:

Amanda L Lloyd,David A Rasko,Harry L T Mobley,

Uropathogenic Escherichia coli (UPEC) strains are responsible for the majority of uncomplicated urinary tract infections, which can present clinically as cystitis or pyelonephritis. UPEC strain CFT073, isolated from the blood of a patient with acute pyelonephritis, was most cytotoxic and most virulent in mice among our strain collection. Based on the genome sequence of CFT073, microarrays were utilized in comparative genomic hybridization (CGH) analysis of a panel of uropathogenic and fecal/commensal E. coli isolates. Genomic DNA from seven UPEC (three pyelonephritis and four cystitis) isolates and three fecal/commensal strains, including K-12 MG1655, was hybridized to the CFT073 microarray. The CFT073 genome contains 5,379 genes; CGH analysis revealed that 2,820 (52.4%) of these genes were common to all 11 E. coli strains, yet only 173 UPEC-specific genes were found by CGH to be present in all UPEC strains but in none of the fecal/commensal strains. When the sequences of three additional sequenced UPEC strains (UTI89, 536, and F11) and a commensal strain (HS) were added to the analysis, 131 genes present in all UPEC strains but in no fecal/commensal strains were identified. Seven previously unrecognized genomic islands (>30 kb) were delineated by CGH in addition to the three known pathogenicity islands. These genomic islands comprise 672 kb of the 5,231-kb (12.8%) genome, demonstrating the importance of horizontal transfer for UPEC and the mosaic structure of the genome. UPEC strains contain a greater number of iron acquisition systems than do fecal/commensal strains, which is reflective of the adaptation to the iron-limiting urinary tract environment. Each strain displayed distinct differences in the number and type of known virulence factors. The large number of hypothetical genes in the CFT073 genome, especially those shown to be UPEC specific, strongly suggests that many urovirulence factors remain uncharacterized.

Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli. Publishing Authors By Initials

AL Lloyd,DA Rasko,HL Mobley,

For similar biological factors: toxins, biological: virulence factors research abstracts see: biological factors: toxins, biological: virulence factors research

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Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 3532-46

Journal Abbreviation:

ISSN: 0021-9193

DAY: 9

MONTH: 03

YEAR: 2007

Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli. Information

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LANGUAGE: eng

NlmUniqueID: 2985120

Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli. Keywords Mesh Terms:

KEYWORDS: Virulence Factors

MESH TERMS: genetics

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Substance Name: Virulence Factors

Registry Number: 0

Grant and Affiliation Information for Defining genomic islands and uropathogen-specific genes in uropathogenic Escherichia coli.

AFFILIATION: Department of Microbiology and Immunology, University of Michigan Medical School, 5641 Medical Science Bldg. II, 1150 West Medical Center Dr., Ann Arbor, MI 48109-0620, USA.

Country: United States

AGENCY: United States NIAID

GRANT: AI059722

ACRONYM: AI

MEDLINETA: J Bacteriol

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