Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants.

Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants. Research Abstract Details 

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Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants. Abstract Text:

J J Hernandez-Ledezma,R W Wright,

The objective of this study was to compare iso-osmolar concentrations (1.5 M) of 1,2-propanediol, glycerol, dimethylsulphoxide and a combination of 1 M propanediol + 0.5M glycerol (PDGLY) as cryoprotectants for murine ovulated oocytes and one-cell embryos. A higher (P < 0.01) percentage of one-cell embryos developed to the two-cell stage when frozen-thawed with 1,2-propanediol (83%) as compared with glycerol (43%), dimethylsulfoxide (51%) or PDGLY (7%). Data recalculated on the basis of two-cell embryos/number of normal one-cell embryos after thawing indicated no differences among single cryoprotectant groups. More (P < 0.01) frozen-thawed, in-vitro fertilized oocytes developed to the two-cell stage when 1,2-propanediol (35%) was used as cryoprotectant as compared with glycerol (15%). Freezing-thawing resulted in a reduced number of two-cell embryos after oocytes were fertilized in-vitro as compared with fresh oocytes. 1,2-propanediol was a better cryoprotectant than glycerol, dimethylsulphoxide or PDGLY for deep freezing of murine oocytes or one-cell embryos.

Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants. Publishing Authors By Initials

JJ Hernandez-Ledezma,RW Wright,

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Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Theriogenology

VOLUME: 32

Page Numbers: 735-43

Journal Abbreviation: Theriogenology

ISSN: 0093-691X

DAY: 26

MONTH: Nov

YEAR: 1989

Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Deep freezing of mouse one-cell embryos and oocytes using different cryoprotectants.

AFFILIATION: Department of Animal Sciences Washington State University Pullman, WA 99164-6332 USA.

Country: United States

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MEDLINETA: Theriogenology

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