Decoration of Pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence.

Decoration of Pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence. Research Abstract Details 

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Decoration of Pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence. Abstract Text:

Marina Harper,Andrew Cox,Frank St Michael,Henrietta Parnas,Ian Wilkie,P J Blackall,Ben Adler,John D Boyce,

Phosphocholine (PCho) is an important substituent of surface structures expressed by a number of bacterial pathogens. Its role in virulence has been investigated in several species, in which it has been shown to play a role in bacterial adhesion to mucosal surfaces, in resistance to antimicrobial peptides, or in sensitivity to complement-mediated killing. The lipopolysaccharide (LPS) structure of Pasteurella multocida strain Pm70, whose genome sequence is known, has recently been determined and does not contain PCho. However, LPS structures from the closely related, virulent P. multocida strains VP161 and X-73 were shown to contain PCho on their terminal galactose sugar residues. To determine if PCho was involved in the virulence of P. multocida, we used subtractive hybridization of the VP161 genome against the Pm70 genome to identify a four-gene locus (designated pcgDABC) which we show is required for the addition of the PCho residues to LPS. The proteins predicted to be encoded by pcgABC showed identity to proteins involved in choline uptake, phosphorylation, and nucleotide sugar activation of PCho. We constructed a P. multocida VP161 pcgC mutant and demonstrated that this strain produces LPS that lacks PCho on the terminal galactose residues. This pcgC mutant displayed reduced in vivo growth in a chicken infection model and was more sensitive to the chicken antimicrobial peptide fowlicidin-1 than the wild-type P. multocida strain.

Decoration of Pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence. Publishing Authors By Initials

M Harper,A Cox,F St Michael,H Parnas,I Wilkie,PJ Blackall,B Adler,JD Boyce,

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Decoration of Pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of bacteriology

VOLUME: 189

Page Numbers: 7384-91

Journal Abbreviation: J. Bacteriol.

ISSN: 0021-9193

DAY: 17

MONTH: 08

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 2985120

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Grant and Affiliation Information for Decoration of Pasteurella multocida lipopolysaccharide with phosphocholine is important for virulence.

AFFILIATION: Australian Research Council Centre of Excellence in Structural and Functional Microbial Genomics, Monash University, VIC, Australia.

Country: United States

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MEDLINETA: J Bacteriol

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