Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells.

Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells. Research Abstract Details 

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Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells. Abstract Text:

Takamitsu Kato,Hatsumi Nagasawa,Christy Warner,Ryuichi Okayasu,Joel S Bedford,

PURPOSE: To determine whether cigarette smoke condensate (CSC) without metabolic activation induces direct DNA double strand breaks (DSB) in the G1 phase of various radiosensitive mutants of CHO cells and whether these breaks display collateral hypersensitivity to CSC with respect to cell killing. MATERIALS & METHODS: We treated the G1-phase cultures of wild-type and DNA repair deficient mutants of CHO cells with various concentrations of CSC and examined the cell survival by colony formation assay and the induction of DNA double strand breaks by constant field gel electrophoresis as well as the phophorylated histone H2-A variant X (gamma-H2AX) assay. RESULTS: Gel analysis and gamma-H2AX focus assay showed significantly fewer, but still detectable levels of DSB per cell after CSC treatment compared to ionizing radiation (IR) exposures, even when equitoxic radiation exposures were delivered at a low dose rate over the same 8-hour exposure used for CSC treatments. None of the three non-homologous end joining (NHEJ) deficient mutants were remarkably hypersensitive to CSC compared to wild-type cells. In contrast, UV-1 cells that are hypersensitive to several base damage and cross-linking agents showed a higher sensitivity to CSC compared to the other CHO cell lines. CONCLUSIONS: DNA DSB produced directly by CSC are not principally responsible for its cytotoxicity. Further, the present study does not rule out the possibility that some of these lesions may secondarily result in DSB, such as may occur during impeded DNA replication and whose repair may require systems other than NHEJ.

Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells. Publishing Authors By Initials

T Kato,H Nagasawa,C Warner,R Okayasu,JS Bedford,

For similar complex mixtures: particulate matter: smoke: tobacco smoke pollution research abstracts see: complex mixtures: particulate matter: smoke: tobacco smoke pollution research

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Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: International journal of radiation biology

VOLUME: 83

Page Numbers: 583-91

Journal Abbreviation: Int. J. Radiat. Biol.

ISSN: 0955-3002

DAY: 3

MONTH: Sep

YEAR: 2007

Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8809243

Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells. Keywords Mesh Terms:

KEYWORDS: Tobacco Smoke Pollution

MESH TERMS: toxicity

Chemical & Substance for Abstract: Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for Cytotoxicity of cigarette smoke condensate is not due to DNA double strand breaks: Comparative studies using radiosensitive mutant and wild-type CHO cells.

AFFILIATION: Department of Environmental and Radiological Health Sciences, Colorado State University, Fort Collins, USA.

Country: England

AGENCY: United States NCI

GRANT: CA96431

ACRONYM: CA

MEDLINETA: Int J Radiat Biol

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