Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells.

Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells. Research Abstract Details 

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Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells. Abstract Text:

S-Y Jeong,S-Y Park,Y-H Kim,M Kim,S-J Lee,

AIMS: The objective of this research was to isolate and identify a cytotoxic marine bacterium, BIT-33, and to investigate the apoptosis effects of its metabolite on colon cancer cells. METHOD AND RESULTS: We isolated 93 marine bacteria from seawater samples. Of these, strain BIT-33 exhibited the strongest cytotoxic activity on three colon cancer cells (HT-29, SW480 and HCT116). Biochemical tests and 16S rDNA sequencing of this strain allowed us to identify BIT-33 as a strain of Bacillus vallismortis. The cytotoxic compound from B. vallismortis BIT-33 was purified by reverse-phase high-performance liquid chromatography. Direct cytotoxic effect of the compound was measured by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide assay. The compound induced apoptosis of colon cancer cells, as indicated by DNA fragmentation of agarose gel electrophoresis, flow cytometric analysis (sub-G(1) method) and annexin V staining. CONCLUSION: The cytotoxic compound from B. vallismortis BIT-33 was purified, and the compound showed direct cytotoxic and apoptotic effects on colon cancer cells in a dose- and time-dependent manner. SIGNIFICANCE AND IMPACT OF THE STUDY: Taken together, our results suggest that the compound from B. vallismortis BIT-33 could be a candidate for the development of apoptosis-specific anti-tumour agents. This study indicated that marine bacteria could be an important source of cytotoxic metabolites.

Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells. Publishing Authors By Initials

SY Jeong,SY Park,YH Kim,M Kim,SJ Lee,

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Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of applied microbiology

VOLUME: 104

Page Numbers: 796-807

Journal Abbreviation: J. Appl. Microbiol.

ISSN: 1365-2672

DAY: 15

MONTH: 11

YEAR: 2007

Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Cytotoxicity and apoptosis induction of Bacillus vallismortis BIT-33 metabolites on colon cancer carcinoma cells.

AFFILIATION: Korea Bio-IT Foundry Center, Pusan National University, Busan, Republic of Korea.

Country: England

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MEDLINETA: J Appl Microbiol

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