Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism.

Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism. Research Abstract Details 

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Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism. Abstract Text:

Tanya D Russell,Carol A Palmer,David J Orlicky,Andreas Fischer,Michael C Rudolph,Margaret C Neville,James L McManaman,

PAT proteins (perilipin, adipophilin, and TIP47) are hypothesized to be critical regulators of lipid accumulation in eukaryotic cells. We investigated the developmental relationships between the expression of these proteins and cytoplasmic lipid droplet (CLD) accumulation in differentiating secretory epithelial cells in mouse mammary glands. Adipophilin (ADPH) specifically localized to CLD in differentiating and lactating mammary glands and was found exclusively in the secreted lipid droplet fraction of mouse milk. ADPH transcripts were selectively detected in secretory epithelial cells, and steady-state levels of both ADPH mRNA and protein increased during secretory differentiation in patterns consistent with functional linkage to CLD accumulation. TIP47 also was detected in secretory epithelial cells; however, it had a diffuse punctate appearance, and its mRNA and protein expression patterns did not correlate with CLD accumulation. Perilipin-positive adipose cells and steady-state levels of perilipin mRNA and protein decreased during mammary gland differentiation, suggesting a progressive loss of adipose lipid storage during this process. Collectively, these data demonstrate that increased ADPH expression is a specialized property of differentiated secretory epithelial cells and provide developmental evidence specifically linking increased ADPH expression to increased CLD accumulation. In addition, evidence is presented that the epithelial and adipose compartments of the mammary gland undergo concerted, developmentally regulated shifts in lipid metabolism that increase the availability of fatty acids necessary for lipid synthesis by milk-secreting cells.

Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism. Publishing Authors By Initials

TD Russell,CA Palmer,DJ Orlicky,A Fischer,MC Rudolph,MC Neville,JL McManaman,

For similar proteins: phosphoproteins research abstracts see: proteins: phosphoproteins research

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Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of lipid research

VOLUME: 48

Page Numbers: 1463-75

Journal Abbreviation: J. Lipid Res.

ISSN: 0022-2275

DAY: 23

MONTH: 04

YEAR: 2007

Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376606

Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism. Keywords Mesh Terms:

KEYWORDS: Phosphoproteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism. Information

Substance Name: Adfp protein, mouse

Registry Number: 148349-60-4

Grant and Affiliation Information for Cytoplasmic lipid droplet accumulation in developing mammary epithelial cells: roles of adipophilin and lipid metabolism.

AFFILIATION: Graduate Programs in Molecular Biology, Department of Obstetrics and Gynecology, University of Colorado Health Sciences Center, Aurora, CO, USA.

Country: United States

AGENCY: United States NICHD

GRANT: R01 HD-045962

ACRONYM: HD

MEDLINETA: J Lipid Res

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