Cytofluorometric quantitation of acridine orange uptake by cultured cells.

Cytofluorometric quantitation of acridine orange uptake by cultured cells. Research Abstract Details 

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Cytofluorometric quantitation of acridine orange uptake by cultured cells. Abstract Text:

The vacuolar accumulation of the lysosomotropic weak base acridine orange (AO) within living cells in culture was studied by cytofluorometry. Mouse peritoneal macrophages, malignant human glioma cells, and normal human glial cells were utilized. Exposure to AO resulted in granular bright red fluorescence, as well as a diffuse weak green background fluorescence. To obtain reproducible "staining" conditions, the red granular fluorescence was measured as a function of dye concentration and staining time. Exposure to high concentrations of AO (greater than 10 micrograms/ml) was found to cause cell damage in combination with markedly changed fluorescence distribution for the cell population with reduced mean fluorescence and increased variability. Granular uptake of AO was pH-dependent and almost zero at pH 5.5. AO fluorescence, as measured by cytofluorometry, was found to be roughly linear to the amount of AO present in the cells, as measured by spectrofluorometry after cell solubilization, indicating negligible fluorescence quenching. AO labelling of living cells might serve as a useful indicator of the condition of the cellular vacuolar (lysosomal) apparatus.

Cytofluorometric quantitation of acridine orange uptake by cultured cells. Publishing Authors By Initials

For similar nervous system: neuroglia research abstracts see: nervous system: neuroglia research

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Cytofluorometric quantitation of acridine orange uptake by cultured cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta pathologica, microbiologica, et immunologica

VOLUME: 92

Page Numbers: 303-9

Journal Abbreviation:

ISSN: 0108-0164

DAY: 26

MONTH: Sep

YEAR: 1984

Cytofluorometric quantitation of acridine orange uptake by cultured cells. Information

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LANGUAGE: eng

NlmUniqueID: 8206622

Cytofluorometric quantitation of acridine orange uptake by cultured cells. Keywords Mesh Terms:

KEYWORDS: Neuroglia

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cytofluorometric quantitation of acridine orange uptake by cultured cells. Information

Substance Name: Acridine Orange

Registry Number: 65-61-2

Grant and Affiliation Information for Cytofluorometric quantitation of acridine orange uptake by cultured cells.

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Country: DENMARK

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MEDLINETA: Acta Pathol Microbiol Immunol

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