Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus.

Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus. Abstract Text:

Y Miura,H Hisaki,S Oda,

The substrate specificity and other properties of a fatty acid hydroxylase system in liver microsomes of the Japanese house musk shrew, Suncus murinus, were examined. Shrew liver microsomes catalyzed the hydroxylation of various saturated fatty acids (C8-C18) to the corresponding omega- and (omega-1)-hydroxy derivatives. The relative activities of the hydroxylase with these substrates were as follows: C12 (100, actual conversion: 7.05 nmol/mg of microsomal protein/min), C10 (90), C14 (87), C16 (23), C8 (19), and C18 (11). The specific activity of the fatty acid hydroxylase in shrew liver was much higher than that in other species. The omega/omega-1-hydroxylation ratio decreased with increasing chain length of fatty acid substrates (C10-C18), but it was 0 for the C8 fatty acid. Both NADPH and O2 were required for hydroxylase activity, and NADH had little effect. The apparent Km value for laurate was 1.6 X 10(-5) M. The hydroxylase activity was 92% inhibited by CO at a CO-O2 ratio of 9. p-Chloromercuribenzoate (0.1 mM) inhibited hydroxylation by 94% whereas iodoacetate (0.1 mM) inhibited it by only 8%. SKF 525-A (1 mM) and menadione (0.01 mM), respectively, caused 41% and 29% inhibition of the activity. It is concluded that the hydroxylase catalyzing fatty acid hydroxylation in shrew liver microsomes is a typical cytochrome P-450-linked monooxygenase.

Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus. Publishing Authors By Initials

Y Miura,H Hisaki,S Oda,

For similar biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research abstracts see: biochemical phenomena, metabolism, and nutrition: biochemical phenomena: substrate specificity research

PUBMED ID PMID:

MEDLINE DATE:

Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 95

Page Numbers: 779-84

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Mar

YEAR: 1984

Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus. Keywords Mesh Terms:

KEYWORDS: Substrate Specificity

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus. Information

Substance Name: Alkane 1-Monooxygenase

Registry Number: EC 1.14.15.3

Grant and Affiliation Information for Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus.

AFFILIATION:

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus Related Publications

• A CLN2-related and thermostable serine-carboxyl proteinase, kumamolysin: cloning, expression, and identification of catalytic serine residue.
• Acidic glycolipids from kidney of suncus (Insectivora).
• C-Reactive protein cutoff-point of 0.65 mg/L may be appropriate not only as a component of metabolic syndrome but also as a risk predictor of cardiovascular disease.
• CEL-I, an Invertebrate N-Acetylgalactosamine-specific C-Type Lectin, Induces TNF-{alpha} and G-CSF Production by Mouse Macrophage Cell Line RAW264.7 Cells.
• Caspase-Independent Cell Death by Fas Ligation in Human Thymus-Derived T Cell Line, HPB-ALL Cells.
• Change in Ucp1 mRNA Expression Following Long-Term Cold Exposure under Normal or High-Fat Diet Regimes in the Cold-Intolerant Mammal, Suncus murinus.
• Cross-talk between the pathways leading to the induction of apoptosis and the secretion of tumor necrosis factor-alpha in ricin-treated RAW 264.7 cells.
• Cytochrome P-450-dependent fatty acid hydroxylase system in Japanese house musk shrew, Suncus murinus.
• Development of an ELISA method for detecting immune complexes between tissue-nonspecific alkaline phosphatase and immunoglobulin G.
• Generation and functional characterization of mice with a disrupted glutathione s-transferase, theta 1 gene.
• Homology of Escherichia coli B glutathione synthetase with dihydrofolate reductase in amino acid sequence and substrate binding site.
• Impaired glucose tolerance, impaired fasting glucose and cardiovascular risk.
• Induction of peroxisomal Lon protease in rat liver after di-(2-ethylhexyl)phthalate treatment.
• Mapping of jog Locus to the Region between D6Mit104 and D6Mit336 on Mouse Chromosome 6.
• Mass spectrometry-based quantitative proteomics.
• Metabolic syndrome and medical expenditure in Japan.
• Mitochondrial oxygen consumption and proton vector.
• Mitochondrial sulfhydryl groups. A possible endogenous probe of conformational changes in the mitochondrial membrane.
• Pepstatin-insenstive acid proteases from Scytalidium lignicolum. Kinetic study with synthetic peptides.
• Phasing of nucleosomes in SV40 chromatin reconstituted in vitro.
• Production of reactive oxygen species (ROS) by various marine fish species during the larval stage.
• Pseudo internal standard approach for label-free quantitative proteomics.
• Retroperitoneal ganglioneuroblastoma resected 8 years after mass screening: a case report.
• Saponin Production in Cell Suspension Cultures of Panax ginseng.
• Selection of lactic yeast producing glucosylceramide from cheese whey.
• Severe hypoglycaemia in a person with insulin autoimmune syndrome accompanied by insulin receptor anomaly type B.
• Tissue-nonspecific alkaline phosphatase with an Asp(289)-->Val mutation fails to reach the cell surface and undergoes proteasome-mediated degradation.
• [Efficacy of dexmedetomidine for controlling delirium in intensive care unit patients]
• [Prefabrication of vascularized bone flap using bone morphogenetic protein (BMP)]
• [The study on Thomsen-Friedenreich antigen in bladder tumor]