Cytochrome C oxidase activity and oxygen tolerance.

Cytochrome C oxidase activity and oxygen tolerance. Research Abstract Details 

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Cytochrome C oxidase activity and oxygen tolerance. Abstract Text:

Jian Li Campian,Xueshan Gao,Mingwei Qian,John W Eaton,

Most cultured cells and intact animals die under hyperoxic conditions. However, a strain of HeLa cells that proliferates under 80% O(2), termed "HeLa-80," has been derived from wildtype HeLa cells ("HeLa-20") by selection for resistance to stepwise increases of oxygen partial pressure. The tolerance of HeLa-80 cells to hyperoxia is not associated with changes in antioxidant defenses or susceptibility to oxidant-mediated killing. Rather, under both 20 and 80% O(2), mitochondrial reactive oxygen species (ROS) production is approximately 2-fold less in HeLa-80 cells, likely related to a significantly higher cytochrome c oxidase (COX) activity ( approximately 2-fold), which may act to deplete upstream electron-rich intermediates responsible for ROS generation. We now report that in HeLa-80 cells elevated COX activity is associated with a >2-fold increase in the regulatory subunit COX Vb, whereas expression levels of other subunits are very close to wild type. Small interfering RNA against Vb selectively lowers COX Vb expression in HeLa-80 cells, increases mitochondrial ROS generation, decreases COX activity 60-80%, and diminishes viability under 80% (but not 20%) O(2). In addition, overexpression of subunit Vb increases COX activity and decreases ROS production in wild-type HeLa-20 cells, along with some increase in tolerance to hyperoxia. Overall, our results indicate that it is possible to make cells tolerant of hyperoxia by manipulation of mitochondrial electron transport. These observations may suggest new pharmaceutical strategies to diminish oxygen-mediated cellular damage.

Cytochrome C oxidase activity and oxygen tolerance. Publishing Authors By Initials

JL Campian,X Gao,M Qian,JW Eaton,

For similar inorganic chemicals: free radicals: reactive oxygen species research abstracts see: inorganic chemicals: free radicals: reactive oxygen species research

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Cytochrome C oxidase activity and oxygen tolerance. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 12430-8

Journal Abbreviation:

ISSN: 0021-9258

DAY: 15

MONTH: 02

YEAR: 2007

Cytochrome C oxidase activity and oxygen tolerance. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Cytochrome C oxidase activity and oxygen tolerance. Keywords Mesh Terms:

KEYWORDS: Reactive Oxygen Species

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cytochrome C oxidase activity and oxygen tolerance. Information

Substance Name: Electron Transport Complex IV

Registry Number: EC 1.9.3.1

Grant and Affiliation Information for Cytochrome C oxidase activity and oxygen tolerance.

AFFILIATION: Molecular Targets Group, James Graham Brown Cancer Center, University of Louisville, Louisville, Kentucky 40202, USA.

Country: United States

AGENCY: United States NIDDK

GRANT: DK58882

ACRONYM: DK

MEDLINETA: J Biol Chem

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