Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species.

Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species. Research Abstract Details 

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Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species. Abstract Text:

Alexander Kunz,Josef Anrather,Ping Zhou,Marcello Orio,Costantino Iadecola,

We sought to determine whether reactive oxygen species (ROS) derived from cyclooxygenase-2 (COX-2) are involved in ischemic brain injury. Focal cerebral ischemia was induced by transient middle cerebral artery occlusion in C57BL/6 mice. The time course of neocortical ROS production was assessed in vivo using hydroethidine as a marker. The same brain sections were used for infarct volume measurements. Transient middle cerebral artery occlusion led to a biphasic increase in ROS production with peaks 2 and 72 h after reperfusion. The COX-2 inhibitor NS398 (10 mg/kg) attenuated the production of COX-2-derived prostaglandin E(2) and reduced brain injury, but did not affect ROS production at 2 and 72 h. Similarly, ROS production was not reduced in COX-2-null mice. In contrast, ROS production and brain injury were reduced in mice lacking the nox2 subunit of the superoxide-producing enzyme nicotinamide adenine dinucleotide phosphate (reduced form) oxidase. The data suggest that COX-2 is not a major source of oxygen radicals after cerebral ischemia and raise the possibility that other COX-2 reaction products, including prostanoids or nonoxygen-based radicals, mediate the COX-2-dependent component of the injury.

Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species. Publishing Authors By Initials

A Kunz,J Anrather,P Zhou,M Orio,C Iadecola,

For similar natural sciences: time: time factors research abstracts see: natural sciences: time: time factors research

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Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of cerebral blood flow and metabolism : of

VOLUME: 27

Page Numbers: 545-51

Journal Abbreviation: J. Cereb. Blood Flow Metab.

ISSN: 0271-678X

DAY: 5

MONTH: 07

YEAR: 2006

Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8112566

Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species. Keywords Mesh Terms:

KEYWORDS: Time Factors

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species. Information

Substance Name: Cyclooxygenase 2

Registry Number: EC 1.14.99.1

Grant and Affiliation Information for Cyclooxygenase-2 does not contribute to postischemic production of reactive oxygen species.

AFFILIATION: Division of Neurobiology, Weill Medical College of Cornell University, New York, NY 10021, USA.

Country: United States

AGENCY: United States NINDS

GRANT: NS35806

ACRONYM: NS

MEDLINETA: J Cereb Blood Flow Metab

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