Cyclic stretch of human lung cells induces an acidification and promotes bacterial growth.

Cyclic stretch of human lung cells induces an acidification and promotes bacterial growth. Research Abstract Details 

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Cyclic stretch of human lung cells induces an acidification and promotes bacterial growth. Abstract Text:

Jérôme Pugin,Irène Dunn-Siegrist,Julien Dufour,Pierre Tissières,Pierre-Emmanuel Charles,Rachel Comte,

The reasons for bacterial proliferation in the lungs of mechanically ventilated patients are poorly understood. We hypothesized that prolonged cyclic stretch of lung cells influenced bacterial growth. Human alveolar type II-like A549 cells were submitted in vitro to prolonged cyclic stretch. Bacteria were cultured in conditioned supernatants from cells submitted to stretch and from control static cells. Escherichia coli had a marked growth advantage in conditioned supernatants from stretched A549 cells, but also from stretched human bronchial BEAS-2B cells, human MRC-5 fibroblasts, and murine RAW 264.7 macrophages. Stretched cells compared with control static cells acidified the milieu by producing increased amounts of lactic acid. Alkalinization of supernatants from stretched cells blocked E. coli growth. In contrast, acidification of supernatants from control cells stimulated bacterial growth. The effect of various pharmacological inhibitors of metabolic pathways was tested in this system. Treatment of A549 cells and murine RAW 264.7 macrophages with the Na(+)/K(+)-ATPase pump inhibitor ouabain during cyclic stretch blocked both the acidification of the milieu and bacterial growth. Several pathogenic bacteria originating from lungs of patients with ventilator-associated pneumonia (VAP) also grow better in vitro at slightly acidic pH (pH 6-7.2), pH similar to those measured in the airways from ventilated patients. This novel metabolic pathway stimulated by cyclic stretch may represent an important pathogenic mechanism of VAP. Alkalinization of the airways may represent a promising preventive strategy in ventilated critically ill patients.

Cyclic stretch of human lung cells induces an acidification and promotes bacterial growth. Publishing Authors By Initials

J Pugin,I Dunn-Siegrist,J Dufour,P Tissières,PE Charles,R Comte,

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Cyclic stretch of human lung cells induces an acidification and promotes bacterial growth. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: American journal of respiratory cell and molecular

VOLUME: 38

Page Numbers: 362-70

Journal Abbreviation: Am. J. Respir. Cell Mol. Biol.

ISSN: 1535-4989

DAY: 5

MONTH: 10

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 8917225

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AFFILIATION: Soins Intensifs, Hôpitaux Universitaires de Genève, 1211 Genève 14, Switzerland. jerome.pugin@medecine.unige.ch

Country: United States

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MEDLINETA: Am J Respir Cell Mol Biol

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