Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells.

Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells. Research Abstract Details 

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Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells. Abstract Text:

Manuel Cevallos,Gordon M Riha,Xinwen Wang,Hui Yang,Shaoyu Yan,Min Li,Hong Chai,Qizhi Yao,Changyi Chen,

The objective of this study was to determine whether cyclic strain could promote human umbilical vein endothelial cells (HUVECs) to express markers in common with the mature smooth muscle cell (SMC) phenotype, suggesting endothelial cell to SMC transdifferentiation. HUVECs were cultured on stretched membranes at 10% stretch and 60 cycles/min for 24-96 hr, and demonstrated elongation with enhanced and organized F-actin distribution. By using real-time polymerase chain reaction analysis, the mRNA levels of five specific SMC markers, SM22-alpha, alpha-smooth muscle actin (alpha-SMA), caldesmon-1, smooth muscle myosin heavy chain (SMMHC), and calponin-1 were significantly increased in cyclic strain-treated HUVECs as compared with those in static control cells. Protein levels of SM22-alpha and alpha-SMA were also substantially increased by Western blot and immunofluorescence staining. In addition, two specific endothelial markers, von Willebrand factor (vWF) and vascular endothelial growth factor receptor-2 (VEGFR-2), showed a reduction in mRNA expression. In addition, cyclic strain-induced increase of SM22-alpha and alpha-SMA expression were reversible when cells were cultured back to the static condition. These results demonstrate a possible endothelial cell to SMC transdifferentiation in response to cyclic strain. Hemodynamic forces in modulating endothelial phenotype may play an important role in the vascular system.

Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells. Publishing Authors By Initials

M Cevallos,GM Riha,X Wang,H Yang,S Yan,M Li,H Chai,Q Yao,C Chen,

For similar proteins: blood proteins: blood coagulation factors: von willebrand factor research abstracts see: proteins: blood proteins: blood coagulation factors: von willebrand factor research

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Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Differentiation; research in biological diversity

VOLUME: 74

Page Numbers: 552-61

Journal Abbreviation: Differentiation

ISSN: 0301-4681

DAY: 3

MONTH: Dec

YEAR: 2006

Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 401650

Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells. Keywords Mesh Terms:

KEYWORDS: von Willebrand Factor

MESH TERMS: genetics

Chemical & Substance for Abstract: Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells. Information

Substance Name: Vascular Endothelial Growth Factor Recep

Registry Number: EC 2.7.1.112

Grant and Affiliation Information for Cyclic strain induces expression of specific smooth muscle cell markers in human endothelial cells.

AFFILIATION: Department of Surgery, Baylor College of Medicine, Houston, TX 77030, USA.

Country: England

AGENCY: United States NCCAM

GRANT: R21 AT003094

ACRONYM: AT

MEDLINETA: Differentiation

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