Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues.

Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Abstract Text:

George C Engelmayr,Virna L Sales,John E Mayer,Michael S Sacks,

Bone marrow-derived mesenchymal stem cells (BMSCs) are relatively accessible and exhibit a pluripotency suitable for cardiovascular applications such as tissue-engineered heart valves (TEHVs). Recently, Sutherland et al. [From stem cells to viable autologous semilunar heart valve. Circulation 2005; 111(21): 2783-91] demonstrated that BMSC-seeded TEHV can successfully function as pulmonary valve substitutes in juvenile sheep for at least 8 months. Toward determining appropriate mechanical stimuli for use in BMSC-seeded TEHV cultivation, we investigated the independent and coupled effects of two mechanical stimuli physiologically relevant to heart valves-cyclic flexure and laminar flow (i.e. fluid shear stress)-on BMSC-mediated tissue formation. BMSC isolated from juvenile sheep were expanded and seeded onto rectangular strips of nonwoven 50:50 blend poly(glycolic acid) (PGA) and poly(l-lactic acid) (PLLA) scaffolds. Following 4 days static culture, BMSC-seeded scaffolds were loaded into a novel flex-stretch-flow (FSF) bioreactor and incubated under static (n=12), cyclic flexure (n=12), laminar flow (avg. wall shear stress=1.1505 dyne/cm(2); n=12) and combined flex-flow (n=12) conditions for 1 (n=6) and 3 (n=6) weeks. By 3 weeks, the flex-flow group exhibited dramatically accelerated tissue formation compared with all other groups, including a 75% higher collagen content of 844+/-278 microg/g wet weight (p<0.05), and an effective stiffness (E) value of 948+/-233 kPa. Importantly, collagen and E values were not significantly different from values measured for vascular smooth muscle cell (SMC) -seeded scaffolds incubated under conditions of flexure alone [Engelmayr et al. The independent role of cyclic flexure in the early in vitro development of an engineered heart valve tissue. Biomaterials 2005; 26(2): 175-87], suggesting that BMSC-seeded TEHV can be optimized to yield results comparable to SMC-seeded TEHV. We thus demonstrated that cyclic flexure and laminar flow can synergistically accelerate BMSC-mediated tissue formation, providing a basis for the rational design of in vitro conditioning regimens for BMSC-seeded TEHV.

Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Publishing Authors By Initials

GC Engelmayr,VL Sales,JE Mayer,MS Sacks,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Biomaterials

VOLUME: 27

Page Numbers: 6083-95

Journal Abbreviation:

ISSN: 0142-9612

DAY: 23

MONTH: 08

YEAR: 2006

Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8100316

Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues.

AFFILIATION: Engineering Tissue Mechanics Laboratory, Department of Bioengineering and the McGowan Institute for Regenerative Medicine, University of Pittsburgh, 100 Technology Drive, Suite 200, Pittsburgh, PA 15219, USA.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Biomaterials

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues Related Publications

• Apolipoprotein CIII-induced THP-1 cell adhesion to endothelial cells involves pertussis toxin-sensitive G protein- and protein kinase C alpha-mediated nuclear factor-kappaB activation.
• Biomechanics of engineered heart valve tissues.
• Cyclic flexure and laminar flow synergistically accelerate mesenchymal stem cell-mediated engineered tissue formation: Implications for engineered heart valve tissues.
• Differences in tissue-remodeling potential of aortic and pulmonary heart valve interstitial cells.
• Drug treatment improves memory in mice.
• Early-life conditions and mechanisms of population health vulnerabilities.
• Effects of decellularization on the mechanical and structural properties of the porcine aortic valve leaflet.
• Endoscopic treatment of fibrous dysplasia confined to the frontal sinus.
• Faculty advisement on theses and dissertations: tips for organizing the process.
• Graft-derived complement as a mediator of transplant injury.
• Guidance of engineered tissue collagen orientation by large-scale scaffold microstructures.
• High-performance, static-coated silicon microfabricated columns for gas chromatography.
• IQGAP1 regulates Salmonella invasion through interactions with actin, Rac1, and Cdc42.
• In-situ deformation of the aortic valve interstitial cell nucleus under diastolic loading.
• Interleukin-10 and the pathogenesis of human visceral leishmaniasis.
• Modified therapeutic community for co-occurring disorders: A summary of four studies.
• On the biaxial mechanical properties of the layers of the aortic valve leaflet.
• Onset of action of azelastine nasal spray compared with mometasone nasal spray and placebo in subjects with seasonal allergic rhinitis evaluated in an environmental exposure chamber.
• Oral health problems and quality of life.
• Post-tonsillectomy bleeding.
• Practice guideline for the performance of physiologic evaluation of extremity arteries.
• Pregnancy plasma glucose levels exceeding the American Diabetes Association thresholds, but below the National Diabetes Data Group thresholds for gestational diabetes mellitus, are related to the risk of neonatal macrosomia, hypoglycaemia and hyperbilirub
• Rapid turnover of apolipoprotein C-III-containing triglyceride-rich lipoproteins contributing to the formation of LDL subfractions.
• Stop-flow programmable selectivity with a dual-column ensemble of microfabricated etched silicon columns and air as carrier gas.
• Synergistic effects of cyclic tension and transforming growth factor-beta1 on the aortic valve myofibroblast.
• The flexural rigidity of the aortic valve leaflet in the commissural region.
• The journal of biomechanical engineering-the next step.
• The relation between collagen fibril kinematics and mechanical properties in the mitral valve anterior leaflet.
• Time-dependent biaxial mechanical behavior of the aortic heart valve leaflet.
• Utility of QuantiFERON-TB Gold in-tube testing for latent TB infection in HIV-infected individuals.