Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes.

Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes. Research Abstract Details 

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Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes. Abstract Text:

J Huang,L R Ballou,K A Hasty,J Huang,L R Ballou,K A Hasty,J Huang,L R Ballou,K A Hasty,J Huang,L R Ballou,K A Hasty,

Mechanical disturbance is directly implicated in the development of osteoarthritis (OA) but the precise mode for degenerative changes is still largely unknown because of the complexity of the biomechanical and biochemical milieu in the articular joint. To investigate the effects of tensile strain on articular cartilage, cyclic equibiaxial tensile strain (CTS, 0.5 Hz, 10% strain) was applied to monolayer cultures of porcine articular chondrocytes by using a Flexercell strain unit. Overproduction of proinflammatory mediators and imbalanced expression of anabolic and catabolic genes were induced. The cellular secretion of nitric oxide (NO) and prostaglandin E(2) (PGE(2)), as well as the mRNA level of cyclooxygenase-2 (COX-2) were up-regulated in response to mechanical stimuli. Additionally, CTS resulted in an initial peak of anabolic response at 3 h of stretch with respect to the expression of type II collagen and aggrecan. After 12 h of CTS, the expression for these two cartilage-specific matrix proteins fell to control levels. A distinct catabolic response developed after 24 h of stretch with an increase in matrix metalloproteinase-1 (MMP-1). Interestingly, a parallel increase in transforming growth factor (TGF) beta3 was associated with the anabolic changes while an increase in expression of TGF beta1, the predominant isoform of the TGF family, appeared at 24 h. The expression at 24 h of MMP-1, an enzyme that degrades interstitial collagens as well as other cartilage matrix proteins and TGF beta1, may signify a shift towards matrix remodeling and potentially a change in matrix composition as a consequence of continuous CTS.

Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes. Publishing Authors By Initials

J Huang,LR Ballou,KA Hasty,J Huang,LR Ballou,KA Hasty,J Huang,LR Ballou,KA Hasty,J Huang,LR Ballou,KA Hasty,

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Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Gene

VOLUME: 404

Page Numbers: 101-9

Journal Abbreviation: Gene

ISSN: 0378-1119

DAY: 14

MONTH: 09

YEAR: 2007

Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes. Information

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LANGUAGE: eng

NlmUniqueID: 7706761

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Grant and Affiliation Information for Cyclic equibiaxial tensile strain induces both anabolic and catabolic responses in articular chondrocytes.

AFFILIATION: Department of Orthopaedic Surgery-Campbell Clinic, University of Tennessee Health Science Center, Memphis, TN 38163, USA; Research Department, Veterans Affairs Medical Center, University of Tennessee Health Science Center, Memphis, TN 38104, USA.

Country: Netherlands

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MEDLINETA: Gene

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