Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat.

Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat. Research Abstract Details 

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Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat. Abstract Text:

S Ono,M Umezaki,N Tojo,S Hashimoto,H Taniyama,T Kaneko,T Fujii,H Morita,C Shimasaki,I Yamazaki,T Yoshimura,T Kato,

Tendamistat is a strong inhibitory protein of porcine pancreatic alpha-amylase (PPA) with a K(i) value of 0.2 nM. To develop potent alpha-amylase inhibitors, we synthesized six odd-length cyclic peptides (5-15 residues) and four even-length cyclic peptides (10 and 12 residues) having the inhibitory sequence of tendamistat. Their PPA inhibitory activities were evaluated, and, among them, the 11-residue cyclic peptide Ten(15-23) (K(i) = 0.27 microM) exhibited the strongest inhibitory activity (K(i) = 0.27-1.41 microM). To examine the effect of cyclic structure on PPA inhibition, ten linear peptides corresponding to the cyclic peptides were also synthesized, and their PPA inhibitory activities were evaluated (K(i) = 0.28-1.00 microM). Interestingly, the 11-residue linear peptide Ten(15-23) exhibited almost the same inhibitory activity (K(i) = 0.28 microM) as that of cyclic Ten(15-23). The results of a circular dichroism study indicated that stabilization of the beta-hairpin structure occurred only for cyclic Ten(15-23). Also, the results of proteolytic digestion experiments of the cyclic and linear Ten(15-23) peptides by trypsin and chymotrypsin suggested no differences in protease resistance between the cyclic and linear structures. Therefore, we demonstrated that both cyclic and linear peptides containing the inhibitory sequence of tendamistat exhibit potent PPA inhibitory activity.

Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat. Publishing Authors By Initials

S Ono,M Umezaki,N Tojo,S Hashimoto,H Taniyama,T Kaneko,T Fujii,H Morita,C Shimasaki,I Yamazaki,T Yoshimura,T Kato,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: amylases: alpha-amylase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: amylases: alpha-amylase research

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Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 129

Page Numbers: 783-90

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: May

YEAR: 2001

Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat. Keywords Mesh Terms:

KEYWORDS: alpha-Amylase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat. Information

Substance Name: Trypsin

Registry Number: EC 3.4.21.4

Grant and Affiliation Information for Cyclic and linear peptides derived from alpha-amylase inhibitory protein tendamistat.

AFFILIATION: Department of System Engineering of Materials and Life Science, Faculty of Engineering, Toyama University, Gofuku, Toyama 930-8555, Japan. shinono@eng.toyama-u.ac.jp

Country: Japan

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MEDLINETA: J Biochem

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