Cyborg lectins: novel leguminous lectins with unique specificities.

Cyborg lectins: novel leguminous lectins with unique specificities. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cyborg lectins: novel leguminous lectins with unique specificities. Abstract Text:

K Yamamoto,I N Maruyama,T Osawa,

Bauhinia purpurea lectin (BPA) is one of the beta-galactose-binding leguminous lectins. Leguminous lectins contain a long metal-binding loop, part of which determines their carbohydrate-binding specificities. Random mutations were introduced into a portion of the cDNA coding BPA that corresponds to the carbohydrate-binding loop of the lectin. An library of the mutant lectin expressed on the surface of lambda foo phages was screened by the panning method. Several phage clones with an affinity for mannose or N-acetylglucosamine were isolated. These results indicate the possibility of making artificial lectins (so-called "cyborg lectins") with distinct and desired carbohydrate-binding specificities.

Cyborg lectins: novel leguminous lectins with unique specificities. Publishing Authors By Initials

K Yamamoto,IN Maruyama,T Osawa,

For similar viruses: virion research abstracts see: viruses: virion research

PUBMED ID PMID:

MEDLINE DATE:

Cyborg lectins: novel leguminous lectins with unique specificities. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 127

Page Numbers: 137-42

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jan

YEAR: 2000

Cyborg lectins: novel leguminous lectins with unique specificities. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 376600

Cyborg lectins: novel leguminous lectins with unique specificities. Keywords Mesh Terms:

KEYWORDS: Virion

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cyborg lectins: novel leguminous lectins with unique specificities. Information

Substance Name: Acetylglucosamine

Registry Number: 7512-17-6

Grant and Affiliation Information for Cyborg lectins: novel leguminous lectins with unique specificities.

AFFILIATION: Laboratory of Molecular Medicine, Department of Integrated Biosciences, Graduate School of Frontier Sciences, The University of Tokyo, Japan. yamamoto@k.u-tokyo.ac.jp

Country: JAPAN

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cyborg lectins: novel leguminous lectins with unique specificities Related Publications

• A chimeric lectin formed from Bauhinia purpurea lectin and Lens culinaris lectin recognizes a unique carbohydrate structure.
• Activation of mouse splenic lymphocyte guanylate cyclase by calcium ion.
• Alpha-casein-binding proteins of guinea pig macrophage membranes and their possible roles in chemotaxis.
• Amination and subsequent derivatization of epoxy-activated agarose for the preparation of new affinity adsorbents.
• Calcium and phosphorus control in patients undergoing hemodialysis: from the view-points of activities of daily living.
• Concanavalin A-induced increase in the membrane fluidity of chicken erythrocytes.
• Conversion of glucose and galactose to lipids by normal and phytohemagglutinin-stimulated lymphocytes.
• Covalently cross-linked monovalent, divalent, and tetravalent derivatives of concanavalin A.
• Cyborg lectins: novel leguminous lectins with unique specificities.
• Heterogeneity of Band 3, the major intrinsic protein of human erythrocyte membranes. Studies by crossed immunoelectrophoresis and crossed immuno-affinoelectrophoresis.
• Identification of enterohemorrhagic Escherichia coli O157-Specific DNA sequence obtained from amplified fragment length polymorphism analysis.
• Isolation of antioxidative phenolic glucosides from lemon juice and their suppressive effect on the expression of blood adhesion molecules.
• Isolation of glycopeptides from the lectin-stimulated human peripheral lymphocyte cell surface.
• Isolation of the antioxidant pyranonigrin-A from rice mold starters used in the manufacturing process of fermented foods.
• Membrane receptors of mouse lymphocytes for various lectins.
• Naturally occurring neuronal NO-synthase inactivators found in Nicotiana tabacum (Solanaceae) and other plants.
• Photoaffinity labeling of concanavalin A. Preparation of a concanavalin A derivative with reduced valence.
• Preparation of monovalent succinyl-concanavalin A and its mitogenic activity.
• Proceedings: Cellular recognition and molecular immunology.
• Purification and characterization of a catalytic subunit of an adenosine 3':5'-monophosphate-dependent protein kinase from human erythrocyte membranes.
• Purification and characterization of beta-N-acetylhexosaminidases and beta-galactosidase from Streptococcus 6646 K.
• Purification of hemagglutinins from Sophora japonica seeds by affinity chromatography.
• Pyrrolizidine Alkaloids from Senecio cruentus.
• Radiotherapy for extramammary Paget's disease: histopathological findings after radiotherapy.
• Stroke rehabilitation in a patient with cerebellar cognitive affective syndrome.
• Studies on the hydrazinolysis of glycoproteins. Core structures of oligosaccharides obtained from Porcine thyroglobulin and pineapple stem bromelain.
• Surgical correction for curly toe using open tenotomy of flexor digitorum brevis tendon.
• Tetrahydrobiopterin protects against guanabenz-mediated inhibition of neuronal nitric-oxide synthase in vitro and in vivo.
• The association between major sialoglycoprotein and spectrin of human erythrocyte membranes as detected by fluorescence resonance energy transfer.
• Two universal primer sets for species identification among vertebrates.