Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells.

Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. Research Abstract Details 

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Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. Abstract Text:

S J Morrison,M Csete,A K Groves,W Melega,B Wold,D J Anderson,

Isolated neural crest stem cells (NCSCs) differentiate to autonomic neurons in response to bone morphogenetic protein 2 (BMP2) in clonal cultures, but these neurons do not express sympathoadrenal (SA) lineage markers. Whether this reflects a developmental restriction in NCSCs or simply inappropriate culture conditions was not clear. We tested the growth and differentiation potential of NCSCs at approximately 5% O(2), which more closely approximates physiological oxygen levels. Eighty-three percent of p75(+)P(0-) cells isolated from embryonic day 14.5 sciatic nerve behaved as stem cells under these conditions, suggesting that this is a nearly pure population. Furthermore, addition of BMP2 plus forskolin in decreased oxygen cultures elicited differentiation of thousands of cells expressing tyrosine hydroxylase, dopamine-beta-hydroxylase, and the SA lineage marker SA-1 in nearly all colonies. Such cells also synthesized and released dopamine and norepinephrine. These data demonstrate that isolated mammalian NCSCs uniformly possess SA lineage capacity and further suggest that oxygen levels can influence cell fate. Parallel results indicating that reduced oxygen levels can also promote the survival, proliferation, and catecholaminergic differentiation of CNS stem cells (Studer et al., 2000) suggests that neural stem cells may exhibit a conserved response to reduced oxygen levels.

Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. Publishing Authors By Initials

SJ Morrison,M Csete,AK Groves,W Melega,B Wold,DJ Anderson,

For similar enzymes and coenzymes: enzymes: oxidoreductases: oxygenases: mixed function oxygenases: tyrosine 3-monooxygenase research abstracts see: enzymes and coenzymes: enzymes: oxidoreductases: oxygenases: mixed function oxygenases: tyrosine 3-monooxygenase research

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Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: The Journal of neuroscience : the official journal

VOLUME: 20

Page Numbers: 7370-6

Journal Abbreviation: J. Neurosci.

ISSN: 1529-2401

DAY: 1

MONTH: Oct

YEAR: 2000

Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. Information

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LANGUAGE: eng

NlmUniqueID: 8102140

Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. Keywords Mesh Terms:

KEYWORDS: Tyrosine 3-Monooxygenase

MESH TERMS: metabolism

Chemical & Substance for Abstract: Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells. Information

Substance Name: Tyrosine 3-Monooxygenase

Registry Number: EC 1.14.16.2

Grant and Affiliation Information for Culture in reduced levels of oxygen promotes clonogenic sympathoadrenal differentiation by isolated neural crest stem cells.

AFFILIATION: Howard Hughes Medical Institute, California Institute of Technology, Pasadena, California 91125, USA.

Country: UNITED STATES

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MEDLINETA: J Neurosci

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