CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges.

CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges. Research Abstract Details 

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CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges. Abstract Text:

Karolina Malanowska,Sumiko Yoneji,Abigail A Salyers,Jeffrey F Gardner,

Although the integrase (IntDOT) of the Bacteroides conjugative transposon CTnDOT has been classified as a member of the tyrosine recombinase family, the reaction it catalyzes appears to differ in some features from reactions catalyzed by other tyrosine recombinases. We tested the ability of IntDOT to cleave and ligate activated attDOT substrates in the presence of mismatches. Unlike other tyrosine recombinases, the results revealed that IntDOT is able to perform ligation reactions even when all the bases within the crossover region are mispaired. We also show that there is a strong bias in the order of strand exchanges during integrative recombination. The top strands are exchanged first in reactions that appear to require 2 bp of homology between the partner sites adjacent to the sites of cleavage. The bottom strands are exchanged next in reactions that do not require homology between the partner sites. This mode of coordination of strand exchanges is unique among tyrosine recombinases.

CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges. Publishing Authors By Initials

K Malanowska,S Yoneji,AA Salyers,JF Gardner,

For similar genetic processes: recombination, genetic research abstracts see: genetic processes: recombination, genetic research

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CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Nucleic acids research

VOLUME: 35

Page Numbers: 5861-73

Journal Abbreviation: Nucleic Acids Res.

ISSN: 1362-4962

DAY: 24

MONTH: 08

YEAR: 2007

CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 411011

CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges. Keywords Mesh Terms:

KEYWORDS: Recombination, Genetic

MESH TERMS: metabolism

Chemical & Substance for Abstract: CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges. Information

Substance Name: Integrases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for CTnDOT integrase performs ordered homology-dependent and homology-independent strand exchanges.

AFFILIATION: Department of Microbiology, University of Illinois, Urbana, IL61801, USA. malanows@uiuc.edu

Country: England

AGENCY: United States NIGMS

GRANT: GM 28717

ACRONYM: GM

MEDLINETA: Nucleic Acids Res

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