Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii.

Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii. Research Abstract Details 

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Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii. Abstract Text:

Nan Li,Bharat K C Patel,Benjamin N Mijts,Kunchithapadam Swaminathan,

This report is the first crystallographic study of an amylase from an organism that is both thermophilic and halophilic. alpha-Amylase from the thermophilic halophile Halothermothrix orenii (AmyA) is a 515-residue protein. It is stable and significantly active at 338 K in starch solution containing NaCl [up to 25%(w/v)]. Purified recombinant AmyA protein crystallizes in the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 55.126, b = 61.658, c = 147.625 A, using the hanging-drop vapour-diffusion method. The crystal diffracts X-rays to a resolution limit of 1.89 A.

Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii. Publishing Authors By Initials

N Li,BK Patel,BN Mijts,K Swaminathan,

For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: amylases: alpha-amylase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: amylases: alpha-amylase research

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Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Acta crystallographica. Section D, Biological crys

VOLUME: 58

Page Numbers: 2125-6

Journal Abbreviation: Acta Crystallogr. D Biol. Crys

ISSN: 0907-4449

DAY: 23

MONTH: 11

YEAR: 2002

Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii. Information

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LANGUAGE: eng

NlmUniqueID: 9305878

Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii. Keywords Mesh Terms:

KEYWORDS: alpha-Amylase

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii. Information

Substance Name: alpha-Amylase

Registry Number: EC 3.2.1.1

Grant and Affiliation Information for Crystallization of an alpha-amylase, AmyA, from the thermophilic halophile Halothermothrix orenii.

AFFILIATION: Laboratory of X-ray Crystallography, Institute of Molecular and Cell Biology, 30 Medical Drive, National University of Singapore, Singapore 117609, Singapore.

Country: Denmark

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MEDLINETA: Acta Crystallogr D Biol Crysta

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