Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013.

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Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013. Abstract Text:

T Inoue,N Nishio,Y Kai,S Harada,Y Ohshiro,S Suzuki,T Kohzuma,S Shidara,H Iwasaki,

New crystals of a blue copper protein, pseudoazurin from denitrifier Achromobacter cycloclastes IAM1013, have been obtained by means of vapor diffusion with ammonium sulfate as a precipitant at pH 6.0 and 4 degrees C. The crystals belong to the orthorhombic system, space group P2(1)2(1)2(1), with unit cell dimensions of a = 56.69(2), b = 61.53(2), and c = 30.20(1) A. The asymmetric unit includes one molecule of pseudoazurin with a Vm value of 2.04 A3/Da. The crystals are so stable against X-ray irradiation that a complete data set up to 1.54 A has been collected using a single native crystal. Solution of the structure was performed by means of the Patterson search techniques, and the current crystallographic R-factor is 17.5% at 3.0 A resolution. Refinement at higher resolution is in progress.

Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013. Publishing Authors By Initials

T Inoue,N Nishio,Y Kai,S Harada,Y Ohshiro,S Suzuki,T Kohzuma,S Shidara,H Iwasaki,

For similar investigative techniques: chemistry, analytical: crystallography: x-ray diffraction: crystallography, x-ray research abstracts see: investigative techniques: chemistry, analytical: crystallography: x-ray diffraction: crystallography, x-ray research

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Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 114

Page Numbers: 761-2

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Dec

YEAR: 1993

Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013. Keywords Mesh Terms:

KEYWORDS: Crystallography, X-Ray

MESH TERMS: isolation & purification

Chemical & Substance for Abstract: Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013. Information

Substance Name: Azurin

Registry Number: 12284-43-4

Grant and Affiliation Information for Crystallization and preliminary X-ray studies on pseudoazurin from Achromobacter cycloclastes IAM1013.

AFFILIATION: Department of Applied Chemistry, Faculty of Engineering, Osaka University.

Country: JAPAN

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MEDLINETA: J Biochem

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