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Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel.

Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel. Research Abstract Details 

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  • Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel. Abstract Text:

    t miyazakiT Miyazaki,t morimotoT Morimoto,k fukuyamaK Fukuyama,h matsubaraH Matsubara,

    The alpha-amylase inhibitor coded 0.19 (0.19AI) from wheat kernel is a dimeric protein which inactivates exogenous alpha-amylases. Crystals of 0.19AI were grown at room temperature and pH 7.0 from a protein solution with a low salt concentration. They were trigonal, belonged to space group P3(1) or P3(2), and had unit cell dimensions of a = b = 79.3 A and c = 60.8 A. The crystals diffract X-rays to at least 2.0 A resolution and are stable to X-ray beams. The asymmetric unit appears to contain two 0.19AI dimers. A potential heavy-atom derivative was prepared by soaking the crystal in a HgCl2 solution.

    Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel. Publishing Authors By Initials

    t miyazakiT Miyazaki,t morimotoT Morimoto,k fukuyamaK Fukuyama,h matsubaraH Matsubara,

    For similar enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: amylases: alpha-amylase research abstracts see: enzymes and coenzymes: enzymes: hydrolases: glycoside hydrolases: amylases: alpha-amylase research

    PUBMED ID PMID:

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    Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel. Journal Published:

    PUBLICATION TYPE: Research Support, Non-U.S. Gov

    Journal: Journal of biochemistry

    VOLUME: 115

    Page Numbers: 179-81

    Journal Abbreviation: J. Biochem.

    ISSN: 0021-924X

    DAY: 19

    MONTH: Feb

    YEAR: 1994

    Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel. Information

    Number of References:

    LANGUAGE: eng

    NlmUniqueID: 376600

    Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel. Keywords Mesh Terms:

    KEYWORDS: alpha-Amylase

    MESH TERMS: antagonists & inhibitors

    Chemical & Substance for Abstract: Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel. Information

    Substance Name: alpha-Amylase

    Registry Number: EC 3.2.1.1

    Grant and Affiliation Information for Crystallization and preliminary X-ray diffraction studies of the alpha-amylase inhibitor coded 0.19 from wheat kernel.

    AFFILIATION: Cereal Food R&D Section, Nisshin Flour Milling Co., Ltd., Saitama.

    Country: JAPAN

    JAPAN Research PublicationJAPAN Research Publication

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    MEDLINETA: J Biochem

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