Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12.

Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12. Research Abstract Details 

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Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12. Abstract Text:

Ji Woo Choi,Jieun Lee,Nishi Kosuke,Che Hun Jung,Jeong Sun Kim,Ji Woo Choi,Jieun Lee,Nishi Kosuke,Che Hun Jung,Jeong Sun Kim,

Nitroreductases that reduce hazardous nitroaromatic compounds are of interest because of their central role in nitroaromatic toxicity, their potential use in bioremediation and their utility in activating prodrugs in directed anticancer therapies. To provide the molecular background to the enzymatic mechanism of the ydjA nitroreductase, which is one of the smallest nitroreductases, the ydjA gene from Escherichia coli K12 was cloned and expressed and the expressed protein Ec_ydjA was purified. Ec_ydjA was crystallized from 20%(w/v) polyethylene glycol 1000, 0.2 M lithium sulfate and 0.1 M phosphate-citrate pH 4.2. Diffraction data were collected to 2.00 A resolution using synchrotron radiation. The crystal belongs to the monoclinic space group C2, with unit-cell parameters a = 87.55, b = 129.28, c = 36.88 A, alpha = 90, beta = 103.8, gamma = 90 degrees . With two Ec_ydjA molecules in the asymmetric unit, the Matthews coefficient was 2.43 A(3) Da(-1) and the solvent content was 48.33%.

Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12. Publishing Authors By Initials

JW Choi,J Lee,N Kosuke,CH Jung,JS Kim,JW Choi,J Lee,N Kosuke,CH Jung,JS Kim,

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Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta crystallographica. Section F, Structural biol

VOLUME: 63

Page Numbers: 1064-6

Journal Abbreviation: Acta Crystallogr. Sect. F Stru

ISSN: 1744-3091

DAY: 30

MONTH: 11

YEAR: 2007

Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Crystallization and preliminary X-ray diffraction analysis of ydjA, a minimal nitroreductase from Escherichia coli K12.

AFFILIATION: Department of Chemistry and Institute of Basic Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.

Country: England

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MEDLINETA: Acta Crystallogr Sect F Struct

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