Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12.

Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12. Research Abstract Details 

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Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12. Abstract Text:

Suk Youl Park,Sang Hak Lee,Jieun Lee,Che Hun Jung,Jeong Sun Kim,Suk Youl Park,Sang Hak Lee,Jieun Lee,Che Hun Jung,Jeong Sun Kim,

The product of the recently discovered ybfF gene, which belongs to the esterase family, does not show high sequence similarity to other esterases. To provide the molecular background to the enzymatic mechanism of the ybfF esterase, the ybfF protein from Escherichia coli K12 (Ec_ybfF) was cloned, expressed and purified. The Ec_ybfF protein was crystallized from 60% Tacsimate and 0.1 M bis-Tris propane buffer pH 7.0. Diffraction data were collected to 1.10 A resolution using synchrotron radiation. The crystal belongs to the orthorhombic space group P2(1)2(1)2(1), with unit-cell parameters a = 66.09, b = 90.71, c = 92.88 A. With two Ec_ybfF molecules in the asymmetric unit, the crystal volume per unit protein weight is 2.17 A(3) Da(-1), corresponding to a solvent content of 42%.

Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12. Publishing Authors By Initials

SY Park,SH Lee,J Lee,CH Jung,JS Kim,SY Park,SH Lee,J Lee,CH Jung,JS Kim,

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Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta crystallographica. Section F, Structural biol

VOLUME: 63

Page Numbers: 1051-3

Journal Abbreviation: Acta Crystallogr. Sect. F Stru

ISSN: 1744-3091

DAY: 30

MONTH: 11

YEAR: 2007

Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12. Information

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LANGUAGE: eng

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Grant and Affiliation Information for Crystallization and preliminary X-ray diffraction analysis of ybfF, a new esterase from Escherichia coli K12.

AFFILIATION: Department of Chemistry and Institute of Basic Sciences, Chonnam National University, Gwangju 500-757, Republic of Korea.

Country: England

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MEDLINETA: Acta Crystallogr Sect F Struct

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