Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin.

Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin. Research Abstract Details 

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Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin. Abstract Text:

Toshio Nakamura,Takashi Tonozuka,Mao Kotani,Kanae Obata,Keiji Oguma,Atsushi Nishikawa,Toshio Nakamura,Takashi Tonozuka,Mao Kotani,Kanae Obata,Keiji Oguma,Atsushi Nishikawa,

HA3, a 70 kDa haemagglutinating protein, is a precursor form of HA3a and HA3b, the subcomponents of Clostridium botulinum type C 16S progenitor toxin. In this report, recombinant HA3 protein was overexpressed in Escherichia coli, purified and crystallized. Diffraction data were collected to 2.6 A resolution and the crystal belonged to the hexagonal space group P6(3). Matthews coefficient and self-rotation function calculations indicate that there is probably one molecule of HA3 in the asymmetric unit. A search for heavy-atom derivatives has been undertaken.

Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin. Publishing Authors By Initials

T Nakamura,T Tonozuka,M Kotani,K Obata,K Oguma,A Nishikawa,T Nakamura,T Tonozuka,M Kotani,K Obata,K Oguma,A Nishikawa,

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Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta crystallographica. Section F, Structural biol

VOLUME: 63

Page Numbers: 1038-40

Journal Abbreviation: Acta Crystallogr. Sect. F Stru

ISSN: 1744-3091

DAY: 21

MONTH: 11

YEAR: 2007

Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin. Information

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LANGUAGE: eng

NlmUniqueID: 101226117

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Grant and Affiliation Information for Crystallization and preliminary X-ray analysis of the HA3 component of Clostridium botulinum type C progenitor toxin.

AFFILIATION: Department of Applied Biological Science, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai-cho, Fuchu-shi, Tokyo 183-8509, Japan.

Country: England

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MEDLINETA: Acta Crystallogr Sect F Struct

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