Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex.

Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex. Research Abstract Details 

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Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex. Abstract Text:

Kinya Nishida,Masataka Horiuchi,Nobuo N Noda,Kiyohiro Takahasi,Norimasa Iwasaki,Akio Minami,Fuyuhiko Inagaki,Kinya Nishida,Masataka Horiuchi,Nobuo N Noda,Kiyohiro Takahasi,Norimasa Iwasaki,Akio Minami,Fuyuhiko Inagaki,

The Tob/BTG family is a group of antiproliferative proteins that contain two highly homologous regions named Box A and Box B. These proteins all associate with CCR4-associated factor 1 (Caf1), which belongs to the ribonuclease D family of deadenylases. The antiproliferative region of human Tob (residues 1-138) and intact hCaf1 were co-expressed in Escherichia coli, purified and successfully cocrystallized. The crystal belongs to the tetragonal space group I422, with unit-cell parameters a = b = 150.9, c = 113.9 A, and is estimated to contain one heterodimer per asymmetric unit. The crystal diffracted to around 2.6 A resolution.

Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex. Publishing Authors By Initials

K Nishida,M Horiuchi,NN Noda,K Takahasi,N Iwasaki,A Minami,F Inagaki,K Nishida,M Horiuchi,NN Noda,K Takahasi,N Iwasaki,A Minami,F Inagaki,

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Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Acta crystallographica. Section F, Structural biol

VOLUME: 63

Page Numbers: 1061-3

Journal Abbreviation: Acta Crystallogr. Sect. F Stru

ISSN: 1744-3091

DAY: 30

MONTH: 11

YEAR: 2007

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LANGUAGE: eng

NlmUniqueID: 101226117

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Grant and Affiliation Information for Crystallization and preliminary crystallographic analysis of the Tob-hCaf1 complex.

AFFILIATION: Department of Structural Biology, Graduate School of Pharmaceutical Science, Hokkaido University, N12, W6, Kita-ku, Sapporo 060-0812, Japan.

Country: England

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MEDLINETA: Acta Crystallogr Sect F Struct

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