Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni.

Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni. Research Abstract Details 

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Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni. Abstract Text:

Ruoyu Gu,Chih-Chia Su,Feng Shi,Ming Li,Gerry McDermott,Qijing Zhang,Edward W Yu,Ruoyu Gu,Chih-Chia Su,Feng Shi,Ming Li,Gerry McDermott,Qijing Zhang,Edward W Yu,

The CmeABC multidrug efflux pump, which belongs to the resistance-nodulation-division (RND) family, recognizes and extrudes a broad range of antimicrobial agents and is essential for Campylobacter jejuni colonization of the animal intestinal tract by mediating the efflux of bile acids. The expression of CmeABC is controlled by the transcriptional regulator CmeR, whose open reading frame is located immediately upstream of the cmeABC operon. To understand the structural basis of CmeR regulation, we have determined the crystal structure of CmeR to 2.2 A resolution, revealing a dimeric two-domain molecule with an entirely helical architecture similar to members of the TetR family of transcriptional regulators. Unlike the rest of the TetR regulators, CmeR has a large center-to-center distance (54 A) between two N termini of the dimer, and a large flexible ligand-binding pocket in the C-terminal domain. Each monomer forms a 20 A long tunnel-like cavity in the ligand-binding domain of CmeR and is occupied by a fortuitous ligand that is identified as glycerol. The binding of glycerol to CmeR induces a conformational state that is incompatible with target DNA. As glycerol has a chemical structure similar to that of potential ligands of CmeR, the structure obtained mimics the induced form of CmeR. These findings reveal novel structural features of a TetR family regulator, and provide new insight into the mechanisms of ligand binding and CmeR regulation.

Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni. Publishing Authors By Initials

R Gu,CC Su,F Shi,M Li,G McDermott,Q Zhang,EW Yu,R Gu,CC Su,F Shi,M Li,G McDermott,Q Zhang,EW Yu,

For similar proteins: transcription factors research abstracts see: proteins: transcription factors research

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MEDLINE DATE:

Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: Journal of molecular biology

VOLUME: 372

Page Numbers: 583-93

Journal Abbreviation: J. Mol. Biol.

ISSN: 0022-2836

DAY: 3

MONTH: 07

YEAR: 2007

Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni. Keywords Mesh Terms:

KEYWORDS: Transcription Factors

MESH TERMS: chemistry

Chemical & Substance for Abstract: Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni. Information

Substance Name: Glycerol

Registry Number: 56-81-5

Grant and Affiliation Information for Crystal structure of the transcriptional regulator CmeR from Campylobacter jejuni.

AFFILIATION: Department of Physics and Astronomy, Iowa State University, Ames, IA 50011, USA.

Country: England

AGENCY: United States NIGMS

GRANT: GM074027

ACRONYM: GM

MEDLINETA: J Mol Biol

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