Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding.

Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding. Abstract Text:

Susanna Saarinen,Hidehito Kato,Takehiko Uchiyama,Tohru Miyoshi-Akiyama,Anastassios C Papageorgiou,Susanna Saarinen,Hidehito Kato,Takehiko Uchiyama,Tohru Miyoshi-Akiyama,Anastassios C Papageorgiou,

Bacterial superantigens are protein toxins with an ability to cause serious diseases in humans by activating a large number of T cells. Streptococcus dysgalactiae-derived mitogen (SDM) is a novel superantigen that is distinct from other known superantigens based on phylogenetic analysis. The X-ray structure of SDM has been determined at 1.95 A resolution. SDM shares the same characteristic fold with other superantigens, but it shows a major structural difference due to the lack of the alpha5 helix between the beta10 and beta11 strands. A bound zinc ion was identified in the structure at the C-terminal domain of the molecule. SDM appears to bind to the major histocompatibility complex class II beta-chain through the zinc-binding site, as described by mutagenesis data and structural comparisons. T-cell binding instead shows a significant difference compared to other superantigens. The mutation of Asn11 (a conserved residue that is known to be significant for T-cell-receptor binding in other superantigens) and Lys15 to Ala did not cause any decrease in the mitogenic activity of SDM. This observation and the lack of the alpha5 helix suggest alterations in T-cell-receptor binding.

Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding. Publishing Authors By Initials

S Saarinen,H Kato,T Uchiyama,T Miyoshi-Akiyama,AC Papageorgiou,S Saarinen,H Kato,T Uchiyama,T Miyoshi-Akiyama,AC Papageorgiou,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of molecular biology

VOLUME: 373

Page Numbers: 1089-97

Journal Abbreviation: J. Mol. Biol.

ISSN: 0022-2836

DAY: 21

MONTH: 08

YEAR: 2007

Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding.

AFFILIATION: Turku Center for Biotechnology, University of Turku and Abo Akademi University, PO Box 123, Tykistökatu 6, BioCity, Turku 20521, Finland.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: 2J4X

Number Hits: 0

Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding Related Publications

• A morphogen gradient model for pattern regulation I. Formation of non-repetitive and repetitive structures.
• A morphogen gradient model for pattern regulation II. Time description of global morphogen formation and field compartmentalization.
• Antioxidant activity of dietary oregano essential oil and alpha-tocopheryl acetate supplementation in long-term frozen stored turkey meat.
• Assessing the feasibility and acceptability of an intervention to reduce anxiety and enhance sensitivity among mothers of very low birth-weight infants.
• Changes of presaccadic cortical activity when performing horizontal, visually guided saccades.
• Chemotherapy with or without anticoagulation as initial management of patients with operable colorectal cancer: a prospective study with at least 5 years follow-up.
• Constituents of Essential Oils from Mentha X rotundifolia Growing Wild in Greece.
• Constituents of Essential Oils from Mentha longifolia Growing Wild in Greece.
• Cortical potentials preceding centrifugal and centripetal self-paced horizontal saccades.
• Cortical potentials with antisaccades.
• Crystal structure of Streptococcus dysgalactiae-derived mitogen reveals a zinc-binding site and alterations in TcR binding.
• Data mining: a new technique in medical research.
• Enterococcus faecalis: an unusual cause of meningitis in a child with non-Hodgkin lymphoma.
• Exit, pursued by a bear.
• Expression, purification and crystallization of Helicobacter pylori L-asparaginase.
• Gadopentetate dimeglumine-enhanced MR in the diagnosis of the Tolosa-Hunt syndrome.
• Month of birth, atopic disease, and sensitization to common aeroallergens in Greece.
• Neural-network methods for boundary value problems with irregular boundaries.
• Octreotide neutralizes dexamethasone antitumor actions on P388 murine lymphocytic leukemia in vivo.
• Particularities of pollen allergies in Greece.
• Preoperative chemotherapy for gastric cancer: a prospective study with at least 1 year follow-up.
• Simultaneous determination of monomeric and oligomeric alkannins and shikonins by high-performance liquid chromatography-diode array detection-mass spectrometry.
• Structural basis of the zinc- and terbium-mediated inhibition of ferroxidase activity in Dps ferritin-like proteins.
• Synergistic interaction between a novel mixed ligand copper(II) chelate complex and a panel of anticancer agents in T47D human breast cancer cells in vitro.
• The incorporation of dehydrated rosemary leaves in the rations of turkeys and their impact on the oxidative stability of the produced raw and cooked meat.
• Triadic communication in the primary care paediatric consultation: a review of the literature.
• Video analysis of communication in paediatric consultations in primary care.
• [Structural changes in bone and bone marrow parenchyma in myelofibrosis.]
• [Study on the short-term treatment of vaginal mycosis with econazole (author's transl)]