Crystal structure of human DGCR8 core.

Crystal structure of human DGCR8 core. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Crystal structure of human DGCR8 core. Abstract Text:

Sun Young Sohn,Won Jin Bae,Jeong Joo Kim,Kyu-Hyeon Yeom,V Narry Kim,Yunje Cho,

A complex of Drosha with DGCR8 (or its homolog Pasha) cleaves primary microRNA (pri-miRNA) substrates into precursor miRNA and initiates the microRNA maturation process. Drosha provides the catalytic site for this cleavage, whereas DGCR8 or Pasha provides a frame for anchoring substrate pri-miRNAs. To clarify the molecular basis underlying recognition of pri-miRNA by DGCR8 and Pasha, we determined the crystal structure of the human DGCR8 core (DGCR8S, residues 493-720). In the structure, the two double-stranded RNA-binding domains (dsRBDs) are arranged with pseudo two-fold symmetry and are tightly packed against the C-terminal helix. The H2 helix in each dsRBD is important for recognition of pri-miRNA substrates. This structure, together with fluorescent resonance energy transfer and mutational analyses, suggests that the DGCR8 core recognizes pri-miRNA in two possible orientations. We propose a model for DGCR8's recognition of pri-miRNA.

Crystal structure of human DGCR8 core. Publishing Authors By Initials

SY Sohn,WJ Bae,JJ Kim,KH Yeom,VN Kim,Y Cho,

For similar proteins research abstracts see: proteins research

PUBMED ID PMID:

MEDLINE DATE:

Crystal structure of human DGCR8 core. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Nature structural & molecular biology

VOLUME: 14

Page Numbers: 847-53

Journal Abbreviation: Nat. Struct. Mol. Biol.

ISSN: 1545-9993

DAY: 19

MONTH: 08

YEAR: 2007

Crystal structure of human DGCR8 core. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101186374

Crystal structure of human DGCR8 core. Keywords Mesh Terms:

KEYWORDS: Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Crystal structure of human DGCR8 core. Information

Substance Name: Proteins

Registry Number: 0

Grant and Affiliation Information for Crystal structure of human DGCR8 core.

AFFILIATION: National Creative Research Center for Structural Biology and Department of Life Science, Pohang University of Science and Technology, Hyo-ja dong, San31, Pohang, KyungBook 790-784, South Korea.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Nat Struct Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: 2YT4

Number Hits: 0

Crystal structure of human DGCR8 core Related Publications

• Alpha-tocopheryl succinate sensitizes human colon cancer cells to exisulind-induced apoptosis.
• Altered membrane dynamics of quantum dot-conjugated integrins during osteogenic differentiation of human bone marrow derived progenitor cells.
• Anatomo-radiological evaluation of lateral approaches to the skull base.
• CDX2 expression is increased in gastric cancers with less invasiveness and intestinal mucin phenotype.
• Conformation-specific recognition of carcinogen-DNA adduct in escherichia coli nucleotide excision repair.
• Ectopic bone formation associated with recombinant human bone morphogenetic proteins-2 using absorbable collagen sponge and beta tricalcium phosphate as carriers.
• Efficient high level expression of peptides and proteins as fusion proteins with the N-terminal domain of L9: application to the villin headpiece helical subdomain.
• Genetic advances in inflammatory bowel disease.
• Illness behavior by the elderly in a korean clan: focus on health-care seeking behavior.
• Inductively-coupled control unit for fully implantable middle ear hearing devices.
• Novel oral formulation of paclitaxel inhibits neointimal hyperplasia in a rat carotid artery injury model.
• Perifollicular fibrosis: pathogenetic role in androgenetic alopecia.
• Preoperative diagnosis of mature cystic teratoma with malignant transformation: analysis of imaging findings and clinical and laboratory data.
• Recent progress in inflammatory bowel disease genetics.
• Regional cerebral perfusion abnormalities in developmental language disorder : Statistical parametric mapping analysis.
• Regulation of mesenchymal stem cell adhesion and orientation in 3D collagen scaffold by electrical stimulus.
• Structural and membrane binding analysis of the Phox homology domain of phosphoinositide 3-kinase-C2alpha.
• Synthesis and antiviral evaluation of novel open-chain analogues of neplanocin A.
• Synthesis of nitric oxide in human osteoblasts in response to physiologic stimulation of electrotherapy.
• TEIN-based Teledermatological Service System among Korea, China and Austria.
• The effect of a fibrin-fibronectin/beta-tricalcium phosphate/recombinant human bone morphogenetic protein-2 system on bone formation in rat calvarial defects.
• Treatment of vertebral artery dissecting aneurysms presenting with progressive myelopathy.
• [A case of rectal schwannoma presenting with hematochezia]
• [A case of splenic pseudocyst difficult to differentiate from splenic lymphangioma]
• [Development of a questionnaire to measure family stress among married working women]
• [Factors associated with success of smoking cessation during 6 months]
• [Factors influencing the emotional state of patients with lymphedema]
• [Magnifying endoscopy in upper gastrointestinal tract]
• [Progress of treatment for bladder cancer]
• [Research progress in SELDI-TOF MS and its clinical applications]