Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein.

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein. Abstract Text:

Galina A Stepanyuk,Zhi-Jie Liu,Svetlana S Markova,Ludmila A Frank,John Lee,Eugene S Vysotski,Bi-Cheng Wang,

Bioluminescence in the sea pansy Renilla involves two distinct proteins, a Ca(2+)-triggered coelenterazine-binding protein (CBP), and Renilla luciferase. CBP contains one tightly bound coelenterazine molecule, which becomes available for reaction with luciferase and O(2) only subsequent to Ca(2+) binding. CBP belongs to the EF-hand superfamily of Ca(2+)-binding proteins and contains three "EF-hand" Ca(2+)-binding sites. The overall spatial structure of recombinant selenomethionine-labeled CBP determined at 1.7 A, is found to approximate the protein scaffold characteristic of the class of Ca(2+)-regulated photoproteins. Photoproteins however, catalyze molecular oxygen addition to coelenterazine producing a 2-hydroperoxycoelenterazine intermediate, which is stabilized within the binding cavity in the absence of Ca(2+). Addition of Ca(2+) triggers the bioluminescence reaction. However in CBP this first step of oxygen addition is not allowed. The different amino acid environments and hydrogen bond interactions within the binding cavity, are proposed to account for the different properties of the two classes of proteins.

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein. Publishing Authors By Initials

GA Stepanyuk,ZJ Liu,SS Markova,LA Frank,J Lee,ES Vysotski,BC Wang,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Photochemical & photobiological sciences : Officia

VOLUME: 7

Page Numbers: 442-7

Journal Abbreviation:

ISSN: 1474-905X

DAY: 19

MONTH: 02

YEAR: 2008

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 101124451

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Crystal structure of coelenterazine-binding protein from Renilla muelleri at 1.7 A: Why it is not a calcium-regulated photoprotein.

AFFILIATION: Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Photochem Photobiol Sci

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Crystal structure of coelenterazine-binding protein from Renilla muelleri at 17 A: Why it is not a calcium-regulated photoprotein Related Publications

• A novel link between the proteasome pathway and the signal transduction pathway of the bone morphogenetic proteins (BMPs).
• Effects of phytoestrogen genistein on delayed after depolarization and triggered activity induced by ouabain in guinea pig papillary muscles.
• Occupational health in China.
• Research on expression of telomerase in human odontogenic lesions.
• Suppression of tumor necrosis factor-mediated apoptosis by nuclear factor kappaB-independent bone morphogenetic protein/Smad signaling.
• [A gene analysis of the low density lipoprotein receptor in Chinese with homozygous familial hyperchol-esterolemia]
• [A novel rat aplastic anemia model induced by 5-fluorouracil combined with busulfan]
• [A study of association between atherothrombotic brain infarction and HLA-DQA1 peptide binding motifs]
• [An experimental study on the bone repairing ability of recombinant human bone morphogenetic protein-2-coral composited artificial bone]
• [Analysis of EEG based on the complexity measure]
• [Apoptosis of bone marrow cells aplastic anemia patients treated with cyclosporin A]
• [Carrier detection and prenatal diagnosis for hemophilia A]
• [Changes of the expression of integrin alpha6beta1 and the activity of focal adhesion kinase in the liver sinusoidal endothelial cell in experimental fibrotic rats]
• [Clinical analysis of 22 patients with primary biliary cirrhosis]
• [Clinical significance of hemostatic molecular markers in acute leukemia]
• [Effects of phenytoin on structural aberration of human sperm chromosomes in vitro]
• [Expression and purification of human papillomavirus type16 L1 protein in a prokaryotic expression system]
• [Extension of cardiac monitoring function by used of ordinary ECG machine]
• [Histopathological features of 27 cases of primary biliary cirrhosis]
• [Multiagent system for individual artificial hip joint]
• [Neomycin inhibits nicotinic receptor-mediated response in pheochromocytoma (PC12) cells]
• [No evidence for association between the alpha 2-macroglobulin polymorphism and Alzheimer's disease in the Han Chinese]
• [PCR cloning of 3'-terminal repeat fragments of Clostridium difficile toxin A and toxin B gene]
• [Preliminary study of prevention from restenosis of hepatic venous in stents by gene transfer of phVEGF165]
• [Prostaglandin inhibitor indomethacin inhibits afferent activities of Adelta and C units in the saphenous nerve of diabetic hyperalgesic rats]
• [Research and development on microwave sterilization]
• [Role of recombinant human bone morphogenetic protein 2/collagen as an onlay bone graft on adult rat calvarial bone]
• [Study of telomerase activity and its related genes expression in leukemia cells]
• [Thrombopoietic effect of recombinant human thrombopoietin gene transferred to mice mediated by electric pulse on normal and experimental thrombocytopenia mice]
• [Thymectomy for late-onset myasthenia gravis in the elderly (with report of 23 cases)]