Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold.

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold. Abstract Text:

Hideshi Yokoyama,Satoshi Fujii,Ikuo Matsui,Hideshi Yokoyama,Satoshi Fujii,Ikuo Matsui,

Stomatin is a major integral membrane protein of human erythrocytes, the absence of which is associated with a form of hemolytic anemia known as hereditary stomatocytosis. However, the function of stomatin is not fully understood. An open reading frame, PH1511, from the hyperthermophilic archaeon Pyrococcus horikoshii encodes p-stomatin, a prokaryotic stomatin. Here, we report the first crystal structure of a stomatin ortholog, the core domain of the p-stomatin PH1511p (residues 56-234 of PH1511p, designated as PhSto(CD)). PhSto(CD) forms a novel homotrimeric structure. Three alpha/beta domains form a triangle of about 50 A on each side, and three alpha-helical segments of about 60 A in length extend from the apexes of the triangle. The alpha/beta domain of PhSto(CD) is partly similar in structure to the band-7 domain of mouse flotillin-2. While the alpha/beta domain is relatively rigid, the alpha-helical segment shows conformational flexibility, adapting to the neighboring environment. One alpha-helical segment forms an anti-parallel coiled coil with another alpha-helical segment from a symmetry-related molecule. The alpha-helical segment shows a heptad repeat pattern, and mainly hydrophobic residues form a coiled-coil interface. According to chemical cross-linking experiments, PhSto(CD) would be able to assemble into an oligomeric form. The coiled-coil fold observed in the crystal probably contributes to self-association.

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold. Publishing Authors By Initials

H Yokoyama,S Fujii,I Matsui,H Yokoyama,S Fujii,I Matsui,

For similar abstracts research abstracts see: abstracts research

PUBMED ID PMID:

MEDLINE DATE:

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of molecular biology

VOLUME: 376

Page Numbers: 868-78

Journal Abbreviation: J. Mol. Biol.

ISSN: 1089-8638

DAY: 8

MONTH: 01

YEAR: 2008

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985088

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold. Keywords Mesh Terms:

KEYWORDS:

MESH TERMS:

Chemical & Substance for Abstract: Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold. Information

Substance Name:

Registry Number:

Grant and Affiliation Information for Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold.

AFFILIATION: School of Pharmaceutical Sciences, University of Shizuoka, Shizuoka 422-8526, Japan.

Country: England

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Mol Biol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER: 3BK6

Number Hits: 0

Crystal structure of a core domain of stomatin from Pyrococcus horikoshii Illustrates a novel trimeric and coiled-coil fold Related Publications

• A multicenter analysis of the FIP1L1-alphaPDGFR fusion gene in Japanese idiopathic hypereosinophilic syndrome: an aberrant splicing skipping the alphaPDGFR exon 12.
• A novel mutation in the SPG3A gene (atlastin) in hereditary spastic paraplegia.
• A novel t(8;18)(q13;q21) in acute monocytic leukemia evolving from constitutional trisomy 8 mosaicism.
• Accurate immobilization of antibody-functionalized peptide nanotubes on protein-patterned arrays by optimizing their ligand-receptor interactions.
• Biofidelity of TRL Legform Impactor and Injury Tolerance of the Human Leg in Lateral Impact.
• Biomimetic and aggregation-driven crystallization route for room-temperature material synthesis: growth of beta-Ga(2)O(3) nanoparticles on peptide assemblies as nanoreactors.
• Chemical genetic screening identifies a novel inhibitor of parallel alignment of cortical microtubules and cellulose microfibrils.
• Chronic myeloid leukemia with a rare variant BCR-ABL translocation: t(9;22;21)(q34;q11.2;q11.2).
• Current issues in pediatric medication adherence.
• Derivative (3)t(3;18)(q27;q21)t(18;16)(q21;?) involving the BCL2 and BCL6 genes in follicular lymphoma with t(3;14;18)(q27;q32;q21).
• Developmentally regulated expression of mil-1 and mil-2, mouse interferon-induced transmembrane protein like genes, during formation and differentiation of primordial germ cells.
• Differences in substrate specificity of C(5)-substituted or C(5)-unsubstituted pyrimidine nucleotides by DNA polymerases from thermophilic bacteria, archaea, and phages.
• Differential chain-length specificities of two isoamylase-type starch-debranching enzymes from developing seeds of kidney bean.
• Effects of diet with or without exercise on leptin and anticoagulation proteins levels in obesity.
• Enhancement patterns and signal-intensity characteristics of small hepatocellular carcinoma in cirrhosis: pathologic basis and diagnostic challenges.
• Estimation of the Type Locality of Hynobius naevius (), a Salamander from Japan (Amphibia: Caudata).
• Fabrication of Au nanowires of uniform length and diameter using a monodisperse and rigid biomolecular template: collagen-like triple helix.
• Hedgehog signaling maintains a tumor stem cell compartment in multiple myeloma.
• Immune pathogenesis of sclerosing cholangitis in biliary atresia.
• Improvement of temporal and dynamic subtraction images on abdominal CT using 3D global image matching and nonlinear image warping techniques.
• MR imaging of hepatocellular carcinomas with biliary tumor thrombi.
• Mineralization of semiconductor nanocrystals on peptide-coated bionanotubes and their pH-dependent morphology changes.
• Modeling of the Changes in Heart Rate and Blood Pressure with Characteristic Respiratory Waveform during Surgery under Local Anesthesia.
• Neuronally released acetylcholine acts on the M2 muscarinic receptor to oppose the relaxant effect of isoproterenol on cholinergic contractions in mouse urinary bladder.
• Potential utility of telmisartan, an angiotensin II type 1 receptor blocker with peroxisome proliferator-activated receptor-gamma (PPAR-gamma)-modulating activity for the treatment of cardiometabolic disorders.
• Roles of the novel interleukin-12-associated cytokine, interleukin-23, in the regulation of T-cell-mediated immunity.
• Roll-over Detection and Sleep Quality Measurement using a Wearable Sensor.
• Serum resistin concentrations in growth hormone-deficient children during growth hormone replacement therapy.
• [Effect of implant junction on bone growth by X-ray analysis]
• [Ten cases of acute superior mesenteric artery occlusion]