Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis.

Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis. Research Abstract Details 

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Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis. Abstract Text:

Debora Lucarelli,Santina Russo,Elspeth Garman,Anna Milano,Wolfram Meyer-Klaucke,Ehmke Pohl,

Members of the ferric/zinc uptake regulator (Fur/Zur) family are the central metal-dependent regulator proteins in many Gram-negative and -positive bacteria. They are responsible for the control of a wide variety of basic physiological processes and the expression of important virulence factors in human pathogens. Therefore, Fur has gathered significant interest as a potential target for novel antibiotics. Here we report the crystal structure of FurB from Mycobacterium tuberculosis at a resolution of 2.7A, and we present biochemical and spectroscopic data that allow us to propose the functional role of this protein. Although the overall fold of FurB with an N-terminal DNA binding domain and a C-terminal dimerization domain is conserved among the Zur/Fur family, large differences in the spatial arrangement of the two domains with respect to each other can be observed. The biochemical and spectroscopic analysis presented here reveals that M. tuberculosis FurB is Zn(II)-dependent and is likely to control genes involved in the bacterial zinc uptake. The combination of the structural, spectroscopic, and biochemical results enables us to determine the structural basis for functional differences in this important family of bacterial regulators.

Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis. Publishing Authors By Initials

D Lucarelli,S Russo,E Garman,A Milano,W Meyer-Klaucke,E Pohl,

For similar inorganic chemicals: elements: metals, heavy: zinc research abstracts see: inorganic chemicals: elements: metals, heavy: zinc research

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Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 9914-22

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 9

MONTH: 01

YEAR: 2007

Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis. Keywords Mesh Terms:

KEYWORDS: Zinc

MESH TERMS: metabolism

Chemical & Substance for Abstract: Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis. Information

Substance Name: Zinc

Registry Number: 7440-66-6

Grant and Affiliation Information for Crystal structure and function of the zinc uptake regulator FurB from Mycobacterium tuberculosis.

AFFILIATION: European Molecular Biology Laboratory, Hamburg Outstation, Notkestrasse 85, D-22603 Hamburg, Germany.

Country: United States

AGENCY: United States NIAID

GRANT: AI15940

ACRONYM: AI

MEDLINETA: J Biol Chem

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