Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method.

Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method. Research Abstract Details 

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Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method. Abstract Text:

PURPOSE: To clarify the developmental capacity of frozen two-cell blastomeres, we investigated in vivo and in vitro viabilities of blastomeres that were frozen ultrarapidly after separation from two-cell mouse embryos. Two-cell embryos obtained from superovulated F1 hybrid females were denuded by treatment with 0.5% pronase solution and then induced to separate into two single blastomeres by gentle pipetting. The blastomeres were cryopreserved by an ultrarapid freezing method. RESULTS: The preimplantation developmental rate of two-cell embryos frozen in 3.0 M DMSO was significantly higher than the rate of those frozen in 15 and 4.5 M DMSO (at least P < 0.05). The in vitro developmental rate of the ultrarapidly frozen-thawed blastomeres separated from two-cell embryos (75.0%) was similar to that of nonfrozen blastomeres (76.0%). When eight pairs of blastocysts that developed from frozen two-cell mouse blastomeres were transferred to pregnant ICR recipients on Day 3, four live singletons were born. CONCLUSION: Thus, the results indicate that two-cell mouse blastomeres can be frozen by the ultrarapid freezing method.

Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method. Publishing Authors By Initials

For similar diagnosis: prognosis: pregnancy outcome research abstracts see: diagnosis: prognosis: pregnancy outcome research

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Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of assisted reproduction and genetics

VOLUME: 11

Page Numbers: 409-13

Journal Abbreviation: J. Assist. Reprod. Genet.

ISSN: 1058-0468

DAY: 17

MONTH: Sep

YEAR: 1994

Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method. Information

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LANGUAGE: eng

NlmUniqueID: 9206495

Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method. Keywords Mesh Terms:

KEYWORDS: Pregnancy Outcome

MESH TERMS: drug effects

Chemical & Substance for Abstract: Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method. Information

Substance Name: Dimethyl Sulfoxide

Registry Number: 67-68-5

Grant and Affiliation Information for Cryopreservation of blastomeres separated from two-cell mouse embryos by an ultrarapid freezing method.

AFFILIATION: Genetic Engineering Research Institute, KIST, Taejon, Korea.

Country: UNITED STATES

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MEDLINETA: J Assist Reprod Genet

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