Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX.

Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. Research Abstract Details 

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Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. Abstract Text:

Michael P Marsh,Samuel K Campos,Matthew L Baker,Christopher Y Chen,Wah Chiu,Michael A Barry,

Recombinant human adenovirus is a useful gene delivery vector for clinical gene therapy. Minor capsid protein IX of adenovirus has been of recent interest since multiple studies have shown that modifications can be made to its C terminus to alter viral tropism or add molecular tags and/or reporter proteins. We examined the structure of an engineered adenovirus displaying the enhanced green fluorescent protein (EGFP) fused to the C terminus of protein IX. Cryoelectron microscopy and reconstruction localized the C-terminal EGFP fusion between the H2 hexon and the H4 hexon, positioned between adjacent facets, directly above the density previously assigned as protein IIIa. The original assignment of IIIa was based largely on indirect evidence, and the data presented herein support the reassignment of the IIIa density as protein IX.

Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. Publishing Authors By Initials

MP Marsh,SK Campos,ML Baker,CY Chen,W Chiu,MA Barry,

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

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Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. Journal Published:

PUBLICATION TYPE: Research Support, U.S. Gov't,

Journal: Journal of virology

VOLUME: 80

Page Numbers: 11881-6

Journal Abbreviation: J. Virol.

ISSN: 0022-538X

DAY: 20

MONTH: 09

YEAR: 2006

Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. Information

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LANGUAGE: eng

NlmUniqueID: 113724

Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: chemistry

Chemical & Substance for Abstract: Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX. Information

Substance Name: Viral Proteins

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Grant and Affiliation Information for Cryoelectron microscopy of protein IX-modified adenoviruses suggests a new position for the C terminus of protein IX.

AFFILIATION: Program in Structural and Computational Biology and Molecular Biophysics, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, TX 77030, USA.

Country: United States

AGENCY: United States NCRR

GRANT: P41 RR 02250

ACRONYM: RR

MEDLINETA: J Virol

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