Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome.

Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome. Research Abstract Details 

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Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome. Abstract Text:

Sukhjit Kaur,Reynald Gillet,Wen Li,Richard Gursky,Joachim Frank,

In eubacterial translation, lack of a stop codon on the mRNA results in a defective, potentially toxic polypeptide stalled on the ribosome. Bacteria possess a specialized mRNA, called transfer messenger RNA (tmRNA), to rescue such a stalled system. tmRNA contains a transfer RNA (tRNA)-like domain (TLD), which enters the ribosome as a tRNA and places an ORF into the mRNA channel. This ORF codes for a signal marking the polypeptide for degradation and ends in a stop codon, leading to release of the faulty polypeptide and recycling of the ribosome. The binding of tmRNA to the stalled ribosome is mediated by small protein B (SmpB). By means of cryo-EM, we obtained a density map for the preaccommodated state of the tmRNA.SmpB.EF-Tu.70S ribosome complex with much improved definition for the tmRNA-SmpB complex, showing two SmpB molecules bound per ribosome, one toward the A site on the 30S subunit side and the other bound to the 50S subunit near the GTPase-associated center. tmRNA is strongly attached to the 30S subunit head by multiple contact sites, involving most of its pseudoknots and helices. The map clarifies that the TLD is located near helix 34 and protein S19 of the 30S subunit, rather than in the A site as tRNA for normal translation, so that the TLD is oriented toward the ORF.

Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome. Publishing Authors By Initials

S Kaur,R Gillet,W Li,R Gursky,J Frank,

For similar bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus: thermus thermophilus research abstracts see: bacteria: gram-negative bacteria: gram-negative aerobic bacteria: gram-negative aerobic rods and cocci: thermus: thermus thermophilus research

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Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Proceedings of the National Academy of Sciences of

VOLUME: 103

Page Numbers: 16484-9

Journal Abbreviation: Proc. Natl. Acad. Sci. U.S.A.

ISSN: 0027-8424

DAY: 20

MONTH: 10

YEAR: 2006

Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7505876

Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome. Keywords Mesh Terms:

KEYWORDS: Thermus thermophilus

MESH TERMS: ultrastructure

Chemical & Substance for Abstract: Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome. Information

Substance Name: RNA, Transfer

Registry Number: 9014-25-9

Grant and Affiliation Information for Cryo-EM visualization of transfer messenger RNA with two SmpBs in a stalled ribosome.

AFFILIATION: Howard Hughes Medical Institute, New York State Department of Health, Empire State Plaza, Albany, NY 12201-0509, USA.

Country: United States

AGENCY: United States NIGMS

GRANT: R37 GM 29169

ACRONYM: GM

MEDLINETA: Proc Natl Acad Sci U S A

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