Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans.

Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. Abstract Text:

Nascimento,Mirian A F Hayashi,Alexandre Kerkis,Vitor Oliveira,Eduardo B Oliveira,Gandhi ,Helena Bonciani Nader,Tetsuo Yamane,Ivarne Luis dos Santos Tersariol,Irina Kerkis,

Recently we have shown that crotamine, a toxin from the South American rattlesnake Crotalus durissus terrificus venom, belongs to the family of cell-penetrating peptides. Moreover, crotamine was demonstrated to be a marker of centrioles, of cell cycle, and of actively proliferating cells. Herein we show that this toxin at non-toxic concentrations is also capable of binding electrostatically to plasmid DNA forming DNA-peptide complexes whose stabilities overcome the need for chemical conjugation for carrying nucleic acids into cells. Interestingly, crotamine demonstrates cell specificity and targeted delivery of plasmid DNA into actively proliferating cells both in vitro and in vivo, which distinguishes crotamine from other known natural cell-penetrating peptides. The mechanism of crotamine penetration and cargo delivery into cells was also investigated, showing the involvement of heparan sulfate proteoglycans in the uptake phase, which is followed by endocytosis and peptide accumulation within the acidic endosomal vesicles. Finally, the permeabilization of endosomal membranes induced by crotamine results in the leakage of the vesicles contents to the cell cytosol.

Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. Publishing Authors By Initials

FD Nascimento,MA Hayashi,A Kerkis,V Oliveira,EB Oliveira,G ,HB Nader,T Yamane,IL Tersariol,I Kerkis,

For similar carbohydrates: polysaccharides: proteoglycans research abstracts see: carbohydrates: polysaccharides: proteoglycans research

PUBMED ID PMID:

MEDLINE DATE:

Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: The Journal of biological chemistry

VOLUME: 282

Page Numbers: 21349-60

Journal Abbreviation: J. Biol. Chem.

ISSN: 0021-9258

DAY: 9

MONTH: 05

YEAR: 2007

Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985121

Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. Keywords Mesh Terms:

KEYWORDS: Proteoglycans

MESH TERMS: metabolism

Chemical & Substance for Abstract: Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans. Information

Substance Name: crotamine

Registry Number: 58740-15-1

Grant and Affiliation Information for Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans.

AFFILIATION: Departamento de Bioquímica, Universidade Federal de São Paulo-Escola Paulista de Medicina, Rua 3 de Maio, 100, Ed. INFAR, CEP 04044-020, São Paulo, Brazil.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Biol Chem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans Related Publications

• Crotamine mediates gene delivery into cells through the binding to heparan sulfate proteoglycans.
• In vitro differentiation of male mouse embryonic stem cells into both presumptive sperm cells and oocytes.
• [Morphological and morphometric research on fibroblasts with genetically determined metabolic defects]