Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.

Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. Abstract Text:

Wnt signaling is involved in developmental processes and in adult stem cell homeostasis. This study analyzes the role(s) of key Wnt signaling mediators in the maintenance and osteogenesis of mesenchymal stem cells (MSCs). We focus specifically on the involvement of low-density lipoprotein-related protein 5 (LRP5), T-cell factor 1 (TCF1), and Frizzled (Fz) receptors, in the presence or absence of exogenous, prototypical canonical (Wnt3a), and non-canonical (Wnt5a) Wnts. In undifferentiated MSCs, LRP5 and TCF1 mediate canonical Wnt signal transduction, leading to increased proliferation, enhanced synergistically by Wnt3a. However, LRP5 overexpression inhibits osteogenic differentiation, further suppressed by Wnt3a. Wnt5a does not affect cell proliferation but enhances osteogenesis of MSCs. Interestingly, Wnt5a inhibits Wnt3a effects on MSCs, while Wnt3a suppresses Wnt5a-mediated enhancement of osteogenesis. Flow cytometry revealed that LRP5 expression elicits differential changes in Fz receptor profiles in undifferentiated versus osteogenic MSCs. Taken together, these results suggest that Wnt signaling crosstalk and functional antagonism with the LRP5 co-receptor are key signaling regulators of MSC maintenance and differentiation.

Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. Publishing Authors By Initials

For similar peptides: intercellular signaling peptides and proteins: wnt proteins research abstracts see: peptides: intercellular signaling peptides and proteins: wnt proteins research

PUBMED ID PMID:

MEDLINE DATE:

Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Intr

Journal: Journal of cellular biochemistry

VOLUME: 101

Page Numbers: 1109-24

Journal Abbreviation: J. Cell. Biochem.

ISSN: 0730-2312

DAY: 1

MONTH: Aug

YEAR: 2007

Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 8205768

Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. Keywords Mesh Terms:

KEYWORDS: Wnt Proteins

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation. Information

Substance Name: Osteocalcin

Registry Number: 104982-03-8

Grant and Affiliation Information for Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.

AFFILIATION: Department and Health and Human Services, Cartilage Biology and Orthopaedics Branch, National Institute of Arthritis, and Musculoskeletal and Skin Diseases, National Institutes of Health, Bethesda, Maryland 20892, USA.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Cell Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation Related Publications

• A facilitated tracking and transcription mechanism of long-range enhancer function.
• A second-generation autologous chondrocyte implantation approach to the treatment of focal articular cartilage defects.
• Activation of p38 and Smads mediates BMP-2 effects on human trabecular bone-derived osteoblasts.
• Biological response of chondrocytes cultured in three-dimensional nanofibrous poly(epsilon-caprolactone) scaffolds.
• Cell density dependent regulation of AP-1 activity is important for chondrogenic differentiation of C3H10T1/2 mesenchymal cells.
• Cellular signaling in developmental chondrogenesis: N-cadherin, Wnts, and BMP-2.
• Chondrogenic differentiation of murine C3H10T1/2 multipotential mesenchymal cells: I. Stimulation by bone morphogenetic protein-2 in high-density micromass cultures.
• Chondrogenic differentiation of murine C3H10T1/2 multipotential mesenchymal cells: II. Stimulation by bone morphogenetic protein-2 requires modulation of N-cadherin expression and function.
• Cross-talk between Wnt signaling pathways in human mesenchymal stem cells leads to functional antagonism during osteogenic differentiation.
• Depression status as a confounder in the effects of antidepressants on the gestation age.
• Fabrication and characterization of six electrospun poly(alpha-hydroxy ester)-based fibrous scaffolds for tissue engineering applications.
• Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem cells.
• Investigation of enablers of knowledge transfer in the medical industry.
• Mechanism of BMP-2 stimulated adhesion of osteoblastic cells to titanium alloy.
• Memory encoded throughout our bodies: molecular and cellular basis of tissue regeneration.
• Mesenchymal stem cell-based cartilage tissue engineering: cells, scaffold and biology.
• Mold-shaped, nanofiber scaffold-based cartilage engineering using human mesenchymal stem cells and bioreactor.
• Murine C3H10T1/2 multipotential cells as an in vitro model of mesenchymal chondrogenesis.
• Regional multilineage differentiation potential of meniscal fibrochondrocytes: implications for meniscus repair.
• Regulation of cartilaginous ECM gene transcription by chondrocytes and MSCs in 3D culture in response to dynamic loading.
• Repair of porcine articular cartilage defect with a biphasic osteochondral composite.
• Simulated joint infection assessment by rapid detection of live bacteria with real-time reverse transcription polymerase chain reaction.
• Spatiotemporal protein distribution of TGF-betas, their receptors, and extracellular matrix molecules during embryonic tendon development.
• TGF-beta1 calcium signaling in osteoblasts.
• Testing and estimation for variable single evoked brain potentials.
• Transforming growth factor-beta3 affects plasminogen activator inhibitor-1 expression in fetal mice and modulates fibroblast-mediated collagen gel contraction.
• Wnt signaling during BMP-2 stimulation of mesenchymal chondrogenesis.
• Wnt-3A enhances bone morphogenetic protein-2-mediated chondrogenesis of murine C3H10T1/2 mesenchymal cells.
• [30 Cases of pulmonary atelectasia during the course of acute broncho-pneumophathies in children.]
• [Primary cancer of the liver in a child 14 months old.]