Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium.

Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Research Abstract Details 

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Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Abstract Text:

Wangzhen Guo,Wei Wang,Baoliang Zhou,Tianzhen Zhang,

Diploid species with a common Gossypium origin are highly diverse in morphology and have been classified into eight genomic groups designated A-G and K. In this study, the transferability of 207 Gossypium arboreum-derived expressed sequence tag-simple sequence repeat (EST-SSR) primer pairs was examined among 25 different diploid accessions representing 7 genomes and 23 Gossypium species. We found that 124 of the 207 (60%) primer pairs produced amplification products in all 25 accessions. The remaining 83 (40%) primer pairs produced amplification in only a subset of species, ranging from 13 to 22 species, which is consistent with some genome- and species-specific amplification. The cross-species amplification of these EST-SSRs in 22 diploid species was 96.5% in 4,554 combinations (207 SSRsx22 species), indicative of a high transferability among the Gossypium species. Furthermore, a high level of polymorphism with an average number of 6.53 alleles per SSR marker was detected. No correlation was found between the repeat motif type and cross-species amplification. DNA sequencing showed that the high-level polymorphism findings was mainly due to changes in the number of repeat motifs and that the high transferability can be attributed to a higher-level conservation in the flanking regions among these diploid Gossypium species. The transferability among these different diploid species presented here can increase the efficiency of transferring genetic information across species and further enhance their introgression into cultivated cotton species by the molecular tagging of important genes existing in these diploid species using the EST-SSR markers.

Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Publishing Authors By Initials

W Guo,W Wang,B Zhou,T Zhang,

For similar biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research abstracts see: biological phenomena, cell phenomena, and immunity: immunity: antibody specificity: species specificity research

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Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 112

Page Numbers: 1573-81

Journal Abbreviation: Theor. Appl. Genet.

ISSN: 0040-5752

DAY: 5

MONTH: 04

YEAR: 2006

Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 145600

Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Keywords Mesh Terms:

KEYWORDS: Species Specificity

MESH TERMS: genetics

Chemical & Substance for Abstract: Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium. Information

Substance Name: Genetic Markers

Registry Number: 0

Grant and Affiliation Information for Cross-species transferability of G. arboreum-derived EST-SSRs in the diploid species of Gossypium.

AFFILIATION: National Key Laboratory of Crop Genetics & Germplasm Enhancement, Cotton Research Institute, Nanjing Agricultural University, 210095 Nanjing, Jiangsu Province, People's Republic of China.

Country: Germany

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MEDLINETA: Theor Appl Genet

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