Cross-linking study on skeletal muscle actin: properties of suberimidate-treated actin.

Cross-linking study on skeletal muscle actin: properties of suberimidate-treated actin. Research Abstract Details 

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Cross-linking study on skeletal muscle actin: properties of suberimidate-treated actin. Abstract Text:

O Ohara,S Takahashi,T Ooi,Y Fujiyoshi,

Cross-linking experiments were performed on muscle skeletal actin, using imidoesters of various chain lengths. Chemical analyses on all products except one (derived from succinimidate) show evidence of the presence of intramolecular cross-links in the molecule. The detailed properties of suberimidate-treated actin (SA) are as follows: SA contains nearly 1 mol of intramolecular cross-link per mol of actin and less than 15% of intermolecularly cross-linked products. Even at a low salt concentration, SA is polymeric, exchanges slowly its bound nucleotide with free nucleotides in solution, and shows an F-actin-type CD spectrum. Electron micrographs of SA reveal that SA exists actually as fibrous polymers in solutions of low ionic strength, although the fibers seem to be less rigid than those at high salt concentration. The F-form of SA at a high salt concentration is indistinguishable from intact F-actin. SA can bind heavy meromyosin and activate the ATPase of heavy meromyosin as observed for intact F-actin. Tropomyosin binds SA only at a high salt concentration. These results show that SA possesses the properties of F-actin even in media of low salt concentration, which are favorable for depolymerization of F-actin. Thus, we may infer that the conformation of SA is frozen in the F-state of actin by the introduction of intramolecular cross-links in the protein.

Cross-linking study on skeletal muscle actin: properties of suberimidate-treated actin. Publishing Authors By Initials

O Ohara,S Takahashi,T Ooi,Y Fujiyoshi,

For similar animals: chordata: vertebrates: mammals: lagomorpha: rabbits research abstracts see: animals: chordata: vertebrates: mammals: lagomorpha: rabbits research

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Cross-linking study on skeletal muscle actin: properties of suberimidate-treated actin. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of biochemistry

VOLUME: 91

Page Numbers: 1999-2012

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Jun

YEAR: 1982

Cross-linking study on skeletal muscle actin: properties of suberimidate-treated actin. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Cross-linking study on skeletal muscle actin: properties of suberimidate-treated actin. Keywords Mesh Terms:

KEYWORDS: Rabbits

MESH TERMS: metabolism

Chemical & Substance for Abstract: Cross-linking study on skeletal muscle actin: properties of suberimidate-treated actin. Information

Substance Name: Adenosine Diphosphate

Registry Number: 58-64-0

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Country: JAPAN

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MEDLINETA: J Biochem

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