Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3.

Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Abstract Text:

Yoko Nakano,Botond Banfi,Algirdas J Jesaitis,Mary C Dinauer,Lee-Ann H Allen,William M Nauseef,

Otoconia are small biominerals in the inner ear that are indispensable for the normal perception of gravity and motion. Normal otoconia biogenesis requires Nox3, a Nox (NADPH oxidase) highly expressed in the vestibular system. In HEK-293 cells (human embryonic kidney cells) transfected with the Nox regulatory subunits NoxO1 (Nox organizer 1) and NoxA1 (Nox activator 1), functional murine Nox3 was expressed in the plasma membrane and exhibited a haem spectrum identical with that of Nox2, the electron transferase of the phagocyte Nox. In vitro Nox3 cDNA expressed an approximately 50 kDa primary translation product that underwent N-linked glycosylation in the presence of canine microsomes. RNAi (RNA interference)-mediated reduction of endogenous p22phox, a subunit essential for stabilization of Nox2 in phagocytes, decreased Nox3 activity in reconstituted HEK-293 cells. p22phox co-precipitated not only with Nox3 and NoxO1 from transfectants expressing all three proteins, but also with NoxO1 in the absence of Nox3, indicating that p22phox physically associated with both Nox3 and with NoxO1. The plasma membrane localization of Nox3 but not of NoxO1 required p22phox. Moreover, the glycosylation and maturation of Nox3 required p22phox expression, suggesting that p22phox was required for the proper biosynthesis and function of Nox3. Taken together, these studies demonstrate critical roles for p22phox at several distinct points in the maturation and assembly of a functionally competent Nox3 in the plasma membrane.

Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Publishing Authors By Initials

Y Nakano,B Banfi,AJ Jesaitis,MC Dinauer,LA Allen,WM Nauseef,

For similar investigative techniques: genetic techniques: gene transfer techniques: transfection research abstracts see: investigative techniques: genetic techniques: gene transfer techniques: transfection research

PUBMED ID PMID:

MEDLINE DATE:

Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Biochemical journal

VOLUME: 403

Page Numbers: 97-108

Journal Abbreviation: Biochem. J.

ISSN: 1470-8728

DAY: 1

MONTH: Apr

YEAR: 2007

Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2984726

Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Keywords Mesh Terms:

KEYWORDS: Transfection

MESH TERMS: metabolism

Chemical & Substance for Abstract: Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3. Information

Substance Name: NADPH Oxidase

Registry Number: EC 1.6.3.1

Grant and Affiliation Information for Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3.

AFFILIATION: Inflammation Program, University of Iowa and Veterans Affairs Medical Center, Iowa City, IA 52241, USA.

Country: England

AGENCY: United States NIAID

GRANT: R01 AI26711

ACRONYM: AI

MEDLINETA: Biochem J

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3 Related Publications

• Activation of the human neutrophil superoxide-generating oxidase: studies in a reconstituted subcellular system.
• Bedeviling details.
• Biological Roles for the NOX Family NADPH Oxidases.
• Critical roles for p22phox in the structural maturation and subcellular targeting of Nox3.
• Diagnostic assays for myeloperoxidase deficiency.
• How human neutrophils kill and degrade microbes: an integrated view.
• Impact of two novel mutations on the structure and function of human myeloperoxidase.
• Isolation and functional analysis of neutrophils.
• Isolation of human neutrophils from venous blood.
• Nox enzymes in immune cells.