Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development.

Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. Research Abstract Details 

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Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. Abstract Text:

Chunxi Ge,Guozhi Xiao,Di Jiang,Renny T Franceschi,

The extracellular signal-regulated kinase (ERK)-mitogen-activated protein kinase (MAPK) pathway provides a major link between the cell surface and nucleus to control proliferation and differentiation. However, its in vivo role in skeletal development is unknown. A transgenic approach was used to establish a role for this pathway in bone. MAPK stimulation achieved by selective expression of constitutively active MAPK/ERK1 (MEK-SP) in osteoblasts accelerated in vitro differentiation of calvarial cells, as well as in vivo bone development, whereas dominant-negative MEK1 was inhibitory. The involvement of the RUNX2 transcription factor in this response was established in two ways: (a) RUNX2 phosphorylation and transcriptional activity were elevated in calvarial osteoblasts from TgMek-sp mice and reduced in cells from TgMek-dn mice, and (b) crossing TgMek-sp mice with Runx2+/- animals partially rescued the hypomorphic clavicles and undemineralized calvaria associated with Runx2 haploinsufficiency, whereas TgMek-dn; Runx2+/- mice had a more severe skeletal phenotype. This work establishes an important in vivo function for the ERK-MAPK pathway in bone that involves stimulation of RUNX2 phosphorylation and transcriptional activity.

Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. Publishing Authors By Initials

C Ge,G Xiao,D Jiang,RT Franceschi,

For similar genetic structures: genome: genome components: genes: transgenes research abstracts see: genetic structures: genome: genome components: genes: transgenes research

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Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. Journal Published:

PUBLICATION TYPE: Research Support, N.I.H., Extr

Journal: The Journal of cell biology

VOLUME: 176

Page Numbers: 709-18

Journal Abbreviation: J. Cell Biol.

ISSN: 0021-9525

DAY: 26

MONTH: Feb

YEAR: 2007

Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 375356

Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. Keywords Mesh Terms:

KEYWORDS: Transgenes

MESH TERMS: enzymology

Chemical & Substance for Abstract: Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development. Information

Substance Name: Mitogen-Activated Protein Kinases

Registry Number: EC 2.7.1.37

Grant and Affiliation Information for Critical role of the extracellular signal-regulated kinase-MAPK pathway in osteoblast differentiation and skeletal development.

AFFILIATION: Department of Periodontics and Oral Medicine, University of Michigan School of Dentistry, Ann Arbor, MI 48109, USA.

Country: United States

AGENCY: United States NIDCR

GRANT: DE12211

ACRONYM: DE

MEDLINETA: J Cell Biol

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