Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product.

Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product. Research Abstract Details 

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Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product. Abstract Text:

Kiminori Miyazaki,Ryoji Nagai,Seikoh Horiuchi,

Pentosidine, a cross-link structure between lysine and arginine residues, is one of the major advanced glycation end products (AGE). It is formed by the reaction of ribose with lysine and arginine. The pentosidine concentration produced by in vitro incubation of plasma obtained from uremic patients was reported to be higher than in normal plasma, indicating that uremic plasma contains an enhancer(s) for pentosidine formation [Miyata, T., Ueda, Y., Yamada, Y., Izuhara, Y., Wada, T., Jadoul, M., Saito, A., Kurokawa, K., and Strihou, C.Y. (1998) J. Am. Soc. Nephrol. 9, 2349-2356]. Since our preliminary study using a monoclonal anti-pentosidine antibody identified creatine as the most effective enhancer, the purpose of the present study was to clarify the mechanism by which creatine contributes to pentosidine formation. Lysine was incubated with ribose in the presence of creatine and analyzed by reverse phase high performance liquid chromatography. A novel fluorescent peak (lambda(ex/em) = 335/385 nm) was detected at 8 min, under conditions at which the authentic pentosidine (lysine was incubated with ribose in the presence of arginine under identical conditions) eluted at 12 min. Structural analyses of this compound revealed a pentosidine-like structure in which the arginine residue was replaced by creatine. This novel AGE-structure, named here creatine-derived pentosidine (C-pentosidine), was detected in the plasma of patients on hemodialysis. These results indicate that creatine increases the formation of C-pentosidine but not authentic pentosidine. This study indicates that creatine plays a direct role as a protein modifier in C-pentosidine formation, although the clinical significance of C-pentosidine is still unknown.

Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product. Publishing Authors By Initials

K Miyazaki,R Nagai,S Horiuchi,

For similar organic chemicals: urea research abstracts see: organic chemicals: urea research

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Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: Journal of biochemistry

VOLUME: 132

Page Numbers: 543-50

Journal Abbreviation: J. Biochem.

ISSN: 0021-924X

DAY: 19

MONTH: Oct

YEAR: 2002

Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product. Information

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LANGUAGE: eng

NlmUniqueID: 376600

Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product. Keywords Mesh Terms:

KEYWORDS: Urea

MESH TERMS: metabolism

Chemical & Substance for Abstract: Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product. Information

Substance Name: Arginine

Registry Number: 74-79-3

Grant and Affiliation Information for Creatine plays a direct role as a protein modifier in the formation of a novel advanced glycation end product.

AFFILIATION: Department of Biochemistry, Kumamoto University School of Medicine, Honjo, Kumamoto 860-0811, Japan.

Country: Japan

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MEDLINETA: J Biochem

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