Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants.

Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Research Abstract Details 

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Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Abstract Text:

W Zhang,S Subbarao,P Addae,A Shen,C Armstrong,V Peschke,L Gilbertson,

After the initial transformation and tissue culture process is complete, selectable marker genes, which are used in virtually all transformation approaches, are not required for the expression of the gene of interest in the transgenic plants. There are several advantages to removing the selectable marker gene after it is no longer needed, such as enabling the reuse of selectable markers and simplifying transgene arrays. We have tested the Cre/ lox system from bacteriophage P1 for its ability to precisely excise stably integrated marker genes from chromosomes in transgenic maize plants. Two strategies, crossing and autoexcision, have been tested and demonstrated. In the crossing strategy, plants expressing the Cre recombinase are crossed with plants bearing a transgene construct in which the selectable marker gene is flanked by directly repeated lox sites. Unlike previous reports in which incomplete somatic and germline excision were common, in our experiments complete somatic and germline marker gene excision occurred in the F(1) plants from most crosses with multiple independent Cre and lox lines. In the autoexcision strategy, the cre gene, under the control of a heat shock-inducible promoter, is excised along with the nptII marker gene. Our results show that a transient heat shock treatment of primary transgenic callus is sufficient for inducing cre and excising the cre and nptII genes. Genetic segregation and molecular analysis confirmed that marker gene removal is precise, complete and stable. The autoexcision strategy provides a way of removing the selectable marker gene from callus or other tissues such as embryos and kernels.

Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Publishing Authors By Initials

W Zhang,S Subbarao,P Addae,A Shen,C Armstrong,V Peschke,L Gilbertson,

For similar plants: plant families and groups: angiosperms: poaceae: zea mays research abstracts see: plants: plant families and groups: angiosperms: poaceae: zea mays research

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Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Journal Published:

PUBLICATION TYPE: Journal Article

Journal: TAG. Theoretical and applied genetics. Theoretisch

VOLUME: 107

Page Numbers: 1157-68

Journal Abbreviation:

ISSN: 0040-5752

DAY: 25

MONTH: 09

YEAR: 2003

Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Information

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LANGUAGE: eng

NlmUniqueID: 145600

Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Keywords Mesh Terms:

KEYWORDS: Zea mays

MESH TERMS: genetics

Chemical & Substance for Abstract: Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants. Information

Substance Name: Integrases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Cre/lox-mediated marker gene excision in transgenic maize (Zea mays L.) plants.

AFFILIATION: Monsanto Company, 700 Chesterfield Parkway North, St. Louis, MO 63017-1732, USA.

Country: Germany

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MEDLINETA: Theor Appl Genet

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