Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells.

Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells. Abstract Text:

We have established H-2D(d)-transgenic (Tg) mice, in which H-2D(d) expression can be extinguished by Cre recombinase-mediated deletion of an essential portion of the transgene (Tg). NK cells adapted to the expression of the H-2D(d) Tg in H-2(b) mice and acquired reactivity to cells lacking H-2D(d), both in vivo and in vitro. H-2D(d)-Tg mice crossed to mice harboring an Mx-Cre Tg resulted in mosaic H-2D(d) expression. That abrogated NK cell reactivity to cells lacking D(d). In D(d) single Tg mice it is the Ly49A+ NK cell subset that reacts to cells lacking D(d), because the inhibitory Ly49A receptor is no longer engaged by its D(d) ligand. In contrast, Ly49A+ NK cells from D(d) x MxCre double Tg mice were unable to react to D(d)-negative cells. These Ly49A+ NK cells retained reactivity to target cells that were completely devoid of MHC class I molecules, suggesting that they were not anergic. Variegated D(d) expression thus impacts specifically missing D(d) but not globally missing class I reactivity by Ly49A+ NK cells. We propose that the absence of D(d) from some host cells results in the acquisition of only partial missing self-reactivity.

Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells. Publishing Authors By Initials

For similar proteins: viral proteins research abstracts see: proteins: viral proteins research

PUBMED ID PMID:

MEDLINE DATE:

Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Journal of immunology (Baltimore, Md. : 1950)

VOLUME: 167

Page Numbers: 6256-62

Journal Abbreviation: J. Immunol.

ISSN: 0022-1767

DAY: 1

MONTH: Dec

YEAR: 2001

Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 2985117

Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells. Keywords Mesh Terms:

KEYWORDS: Viral Proteins

MESH TERMS: physiology

Chemical & Substance for Abstract: Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells. Information

Substance Name: Integrases

Registry Number: EC 2.7.7.-

Grant and Affiliation Information for Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells.

AFFILIATION: Ludwig Institute for Cancer Research, University of Lausanne, Epalinges, Switzerland.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: J Immunol

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Cre recombinase-mediated inactivation of H-2Dd transgene expression: evidence for partial missing self-recognition by Ly49A NK cells Related Publications

• Effects of heavy ions and energetic protons on normal human fibroblasts.
• Effects of orthoses on upright functional skills of children and adolescents with cerebral palsy.
• Identification of DNA Methylation in 3' Genomic Regions that are Associated with Upregulation of Gene Expression in Colorectal Cancer.
• Long-term, multilineage hematopoiesis occurs in the combined absence of -catenin and {gamma}-catenin.
• Not Available
• The time dependence of bystander responses induced by iron-ion radiation in normal human skin fibroblasts.
• [1000 plastic operations; experiences and results.]
• [5 Year results of uterus carcinoma treatment (1940-1947) at the gynecological clinic in St. Gallen.]
• [Cesarean delivery under lumbar anesthesia with nupercaine.]
• [Direct injection sub operationem of malignant tumors and areas of neoplastic infiltration with colloidal radioactive gold, Au198.]
• [Microscopical appearance of atypical & abnormal cervical squamous epithelium in states of involution.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [Not Available.]
• [OBSERVATION ON MATERNAL MORTALITY AT THE GYNECOLOGICAL CLINIC OF THE UNIVERSITY OF ZUERICH DURING 1951-1963.]
• [Possibilities of multimedia online teaching in medical education]
• [Preliminary experiences with dibutil in therapy of the Parkinson syndrome.]
• [Prothrombin and factor 7 content of blood at the end of pregnancy and in newborn.]
• [Pulmonary gas embolism in laparoscopy--a rare complication]
• [Results of 10 years of performing abortions at the Gynecologic Clinic of the Dresden Medical Academy]
• [Significance of vaginal hysterectomy in surgical therapy of prolapse.]
• [The behavior of central venous pressure and acid-base balance in gynecologic laparoscopy]
• [Therapy of urinary incontinence in women.]
• [Ureteral and vesico-vaginal fistula after radical abdominal hysterectomy.]
• [Weaning statistics according to data of the St. Galler maternity hospital.]
• [When and how may we operate on cervical carcinoma?]
• [pH and CO2 determination in human amniotic fluid (normal range between the 36th and 41st weeks).]