CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells.

CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Research Abstract Details 

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CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Abstract Text:

Jennifer Huggins,Tina Pellegrin,Raymond E Felgar,Chungwen Wei,Miguel Brown,Bo Zheng,Eric C B Milner,Steven H Bernstein,Ignacio Sanz,Martin S Zand,

Unmethylated CpG DNA activation of naive CD27- B cells has been reported to require B-cell-receptor (BCR) cross-linking. We describe a culture system using CpG DNA with sequential steps for T-cell-independent activation of naive CD19+CD27- human peripheral blood B cells that induces efficient CD138+ plasma-cell differentiation. CD27+ and CD27- B cells were cultured in a 3-step system: (1) days 0 to 4: CpG, IL-2/10/15; (2) days 4 to 7: IL-2/6/10/15 and anti-CD40L; (3) days 7 to 10: IL-6/15, IFN-alpha, hepatocyte growth factor, and hyaluronic acid. Both CD27+ and CD27- B cells up-regulated intracytoplasmic TLR-9 following CpG DNA activation. CD27- B-cell activation required cell-cell contact. Both naive and memory B cells progressed to a plasma-cell phenotype: CD19lowCD20lowCD27+CD38+HLA-DRlow. Seventy percent of the CD27--derived CD138+ cells demonstrated productive V chain rearrangements without somatic mutations, confirming their origin from naive precursors. Plasma cells derived from CD27+ B cells were primarily IgG+, while those from CD27- B cells were IgM+. Our results indicate that under certain conditions, naive B cells increase TLR-9 expression and proliferate to CpG DNA stimulation without BCR signaling. In addition to its immunologic significance, this system should be a valuable method to interrogate the antigenic specificity of naive B cells.

CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Publishing Authors By Initials

J Huggins,T Pellegrin,RE Felgar,C Wei,M Brown,B Zheng,EC Milner,SH Bernstein,I Sanz,MS Zand,

For similar proteins: dna-binding proteins: toll-like receptor 9 research abstracts see: proteins: dna-binding proteins: toll-like receptor 9 research

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CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Blood

VOLUME: 109

Page Numbers: 1611-9

Journal Abbreviation: Blood

ISSN: 0006-4971

DAY: 10

MONTH: 10

YEAR: 2006

CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 7603509

CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Keywords Mesh Terms:

KEYWORDS: Toll-Like Receptor 9

MESH TERMS: cytology

Chemical & Substance for Abstract: CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells. Information

Substance Name: DNA

Registry Number: 9007-49-2

Grant and Affiliation Information for CpG DNA activation and plasma-cell differentiation of CD27- naive human B cells.

AFFILIATION: Department of Pathology, James P. Wilmot Cancer Center, University of Rochester Medical Center, NY 14642, USA.

Country: United States

AGENCY: United States NIAID

GRANT: U19 AI56390

ACRONYM: AI

MEDLINETA: Blood

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