Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization.

Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization. Research Abstract Details 

Research Abstract Table of Contents

Jump to the:

Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization. Abstract Text:

Xiaohui Hou,Bailing Liu,Xiaobo Deng,Baotan Zhang,Hualin Chen,Rong Luo,

In this study, micron-sized poly(styrene-co-glycidyl methacrylate) (PSt-GMA) fluorescent microspheres of 5.1microm in diameter were synthesized via dispersion polymerization of styrene and glycidyl methacrylate in the presence of 1,4-bis(5-phenyloxazol-2-yl) benzene (POPOP), which provided surface functional groups for covalent immobilization of enzymes. In an effort to study the biocompatibility of the microspheres' surface, glucose oxidase and beta-d-(+)-glucose were selected as a catalytic system for enzymatic assays. A colorimetric method was adopted in evaluating enzymatic activity by introducing horseradish peroxidase (HRP). Both the immobilization amount and the apparent activity of immobilized glucose oxidase from Aspergillus niger (GOD) were determined at different conditions. The results show that the immobilized enzymes retained approximately 28 to 34% activity, as compared with free enzymes, without pronounced alteration of the optimum pH and temperature. Kinetics studies show that the corresponding values of K(m) and V(max) are 23.2944 mM and 21.6450M/min.mg GOD for free enzymes and 35.1780 mM and 15.4799M/min.mg GOD for immobilized enzymes. The operational stability studies show that immobilized GOD could retain nearly 50% initial activity after being washed 20 times. The results suggest that the resultant PSt-GMA fluorescent microspheres provide a suitable surface for covalent immobilizing biomolecules; therefore, they have the potential of being used in fluorescence-based immunoassays in high-throughput screening or biosensors.

Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization. Publishing Authors By Initials

X Hou,B Liu,X Deng,B Zhang,H Chen,R Luo,

For similar environment and public health: environment: environment, controlled: temperature research abstracts see: environment and public health: environment: environment, controlled: temperature research

PUBMED ID PMID:

MEDLINE DATE:

Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization. Journal Published:

PUBLICATION TYPE: Research Support, Non-U.S. Gov

Journal: Analytical biochemistry

VOLUME: 368

Page Numbers: 100-10

Journal Abbreviation: Anal. Biochem.

ISSN: 0003-2697

DAY: 27

MONTH: 04

YEAR: 2007

Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization. Information

Number of References:

LANGUAGE: eng

NlmUniqueID: 370535

Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization. Keywords Mesh Terms:

KEYWORDS: Temperature

MESH TERMS: chemistry

Chemical & Substance for Abstract: Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization. Information

Substance Name: Horseradish Peroxidase

Registry Number: EC 1.11.1.-

Grant and Affiliation Information for Covalent immobilization of glucose oxidase onto poly(styrene-co-glycidyl methacrylate) monodisperse fluorescent microspheres synthesized by dispersion polymerization.

AFFILIATION: Chengdu Institute of Organic Chemistry, Chinese Academy of Sciences, Chengdu 610041, People's Republic of China.

Country: United States

AGENCY:

GRANT:

ACRONYM:

MEDLINETA: Anal Biochem

REFSOURCE:

DATABASENAME:

ACCESSION NUMBER:

Number Hits: 0

Covalent immobilization of glucose oxidase onto polystyrene-co-glycidyl methacrylate monodisperse fluorescent microspheres synthesized by dispersion polymerization Related Publications

• Development of wiring specificity of the Drosophila olfactory system.
• Diagnosis and differential diagnosis of nodular lymphocyte-predominant Hodgkin's lymphoma.
• Ski represses bone morphogenic protein signaling in Xenopus and mammalian cells.
• Testing the amyloid toxicity hypothesis of Alzheimer's disease in transgenic Caenorhabditis elegans model.
• [A reprot of 2-phase clinical trioe of Bing Shi Yu Shang ointment for the treatment of partial thickness burn]
• [Aberrant expression of MUC2 and MUC3 genes in gastric carcinoma and its significance]
• [Absence of evidence for the association of single nucleotide polymorphisms in Annexin A1 gene with type 2 diabetes in Chinese]
• [Advances in burn immune research]
• [An experimental study of the intracellular distribution and the activation of mitogen-activated protein kinases in myocardial cells in scalded rats]
• [Change of nuclear factor-kappaB activity in early burn injury and its significance]
• [Changes of sarco(endo)plasmic reticulum Ca2+-Mg2+-ATPase activity of cultured myoblast induced by cyclic stretch]
• [Clinical study of subcutaneous administration of gonadotropin-releasing hormone agonist every six weeks in girls with idiopathic central precocious puberty]
• [Clinical study on the postburn change in the hypothalamus-pituitary-adrenal hormones in severely burned patients]
• [Combination of mycophenolate mofetil with cyclosporine A and methotrexate as acute GVHD prophylaxis after unrelated donor allogeneic bone marrow transplantation]
• [Early scale effects of the visual spatial attention]
• [Effect of different infusion volume on hemodynamics of portal hypertension canines after hemorrhagic shock]
• [Evaluation of capillary chromatographic columns packed by electrokinetic packing method]
• [Gene expression of matrix mettallo-proteinase 14 in laryngeal squamous cell carcinoma]
• [High performance liquid chromatographic analysis of organic acids in foods]
• [In vitro expression of wild type and precore mutant woodchuck hepatitis virus]
• [Influence of clenbuterol on the sensory neurotrophism of denervated red and white muscles]
• [Inhibition of momordica anti-HIV protein of MAP30 on HBeAg expression by laser scanning confocal microscopy]
• [Pharmacognostical identification of Polygonum perfoliatum]
• [Studies on calluses induced from various explants of Cistanche deserticola]
• [Study of genes related with kidney deficiency syndrome]
• [Study on inhibitory effect of ampelopsin on melanoma by serologic pharmacological method]
• [Study on separating characteristics in capillary reversed-phase electrochromatography]
• [Study on the correlation of the biochemical indexes in flouride workers]
• [The research of assemblying animal models of keloid employing the method of tissue engineering]
• [Tumor necrosis factor and nitric oxide in the protective heat stress model]